Validation of non-invasive morphological and diffusion imaging in mouse emphysema by micro-CT and hyperpolarized ^129Xe MRI
Validation of non-invasive morphological and diffusion imaging in mouse emphysema by micro-CT and hyperpolarized ^129Xe MRI
复制标题
通过显微 CT 和超极化^129Xe MRI 对小鼠肺气肿进行无创形态学和扩散成像验证
DOI：
10.1165/rcmb.2012-0350oc
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
et al.
中科院分区：
文献类型：
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作者：
Satoshi Tetsumoto;Yoshioto Takeda;Hirohiko Imai;et al.
动物疾病模型是研究肺气肿发病机制和开发新药的关键，但评价小鼠肺气肿模型的方法有效性和敏感性有限。在这项研究中，我们评估了超极化129磁共振成像（MRI）和微型计算机断层扫描（micro-CT）与传统方法，如体积描记术和组织学。弹性蛋白酶处理的小鼠和脂联素敲除小鼠被用作鼠肺气肿模型以评估这些模式。弹性蛋白酶给药后3周，根据平均线性截距和体积描记参数评价显著和异质性肺气肿。值得注意的是，通过micro-CT检查，低密度区域的分布与全肺的平均线性截距和体积描记参数相关。这些相关性也在区域性地区观察到。此外，我们还介绍了超极化129磁共振成像，它可以评估自主呼吸时肺泡和血液之间的气体交换。弹性蛋白酶处理的小鼠的气体交换（fD）和肺泡大小（Vs/Va）的参数显着降低，并与体积描记参数中度相关。重要的是，我们可以用micro-CT检测到低密度区fD值的降低，这表明肺气肿病变的气体交换减少。同样，这些参数（fD和Vs/Va）也降低脂联素敲除小鼠，表现出肺气肿均匀分布。我们证明了129 MRI和micro-CT结合传统模式的可行性。这些无创模式提供了补充数据，可用于重复估计区域气体交换和肺形态。
Animal disease models are pivotal in investigating the pathogenesis of emphysema and developing novel drugs, but the modalities to evaluate murine emphysema models have been of limited validity and sensitivity. In this study, we evaluated hyperpolarized129Xe magnetic resonance imaging (MRI) and micro–computed tomography (micro-CT) compared with traditional methods, such as plethysmography and histology. Elastase-treated mice and adiponectin knockout mice were used as murine emphysema models to evaluate these modalities. Three weeks after elastase administration, significant and heterogeneous emphysema was evaluated according to the mean linear intercept and plethysmography parameters. Notably, the distribution of low-density areas, as examined by micro-CT, correlated with the mean linear intercept and plethysmography parameters in whole lungs. These correlations were also observed in regional areas. Furthermore, we introduced hyperpolarized129Xe MRI, which can evaluate gas exchange between the alveoli and blood during spontaneous breathing. Parameters of gas exchange (fD) and alveolar size (Vs/Va) were significantly decreased in elastase-treated mice, and moderately correlated with the plethysmography parameters. Of importance, we could detect a decrease of the fD value in low-density areas with micro-CT, suggesting that gas exchange decreased in emphysematous lesions. Likewise, these parameters (fD and Vs/Va) were also decreased in adiponectin knockout mice, which exhibit emphysema with a homogeneous distribution. We demonstrated the feasibility of129Xe MRI and micro-CT in combination with traditional modalities. These noninvasive modalities provide complementary data that can be used for repeated estimations of regional gas exchange and lung morphology.