MONITORING LIPID-PEROXIDATION BY BREATH ANALYSIS - ENDOGENOUS HYDROCARBONS AND THEIR METABOLIC ELIMINATION
MONITORING LIPID-PEROXIDATION BY BREATH ANALYSIS - ENDOGENOUS HYDROCARBONS AND THEIR METABOLIC ELIMINATION
复制标题
DOI：
10.1016/0041-008x(80)90066-6
复制
发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
REMMER, H
中科院分区：
文献类型：
--
作者：
FRANK, H;HINTZE, T;REMMER, H
越来越多的实验室将监测呼吸中的乙烷和戊烷作为一种非侵入性方法来测量脂质过氧化作用。烷烃通过多不饱和脂肪酸的过氧化分解产生。禁食的Sprague-Dawley大鼠以约1.7 nmol/kg/h的速率呼出这些烃。通过改进的分析程序，可以在动物的呼吸中检测到其他挥发性碳氢化合物：乙烯（每小时1.1纳摩尔/千克）、丙烷（每小时0.7纳摩尔/千克）、正丁烷（每小时0.7纳摩尔/千克）、异戊烷和异丁烯。如果动物被关在一个封闭的系统中，呼出的碳氢化合物会在大气中积累。由于再摄取和随后的肝脏代谢，增加不是线性的，而是接近稳态浓度。碳氢化合物被肝单加氧酶氧化，其氧化速率随其分子量的增加而增加。在封闭系统中，大鼠对乙烷、丙烷、丁烷、戊烷和异丁烯的消除也有相同的结果。代谢受到多种物质的抑制：二硫威、乙醇和四氢呋喃。在用疑似诱导脂质过氧化的化合物处理后，碳氢化合物释放到气相中的增加可能是由于代谢降低。如果在乙醇给药后碳氢化合物呼气仅中度升高，则必须考虑这一方面。过氧化微粒体表现出升高的碳氢化合物输出时，处理与CO，以阻止代谢。这项研究提供了一个程序的歧视的抑制和过氧化作用，这是很重要的，如果增加超过对照水平只是中度。它可以作为实验室动物代谢能力的监测器。
Monitoring of ethane and pentane in breath as a noninvasive method to measure lipid peroxidation is performed by an increasing number of laboratories. The alkanes are generated through peroxidative breakdown of polyunsaturated fatty acids. Fasted Sprague-Dawley rats exhaled these hydrocarbons at a rate of approximately 1.7 nmol/kg per h. Through an improved analytical procedure other volatile hydrocarbons could be detected in the breath of animals: ethene (1.1 nmol/kg per h), propane (0.7 nmol/kg per h), n-butane (0.7 nmol/kg per h), iso-pentane and iso-butene. Exhaled hydrocarbons accumulated in atmosphere if an animal was confined in a closed system. The increase was not linear but a steady-state concentration was approached, due to reuptake and subsequent hepatic metabolism. Hydrocarbons were oxidized by hepatic monooxygenases, at rates increasing with their molecular masses. The same was found for elimination of ethane, propane, butane, pentane and iso-butene by a rat from closed system. Metabolism was inhibited by a variety of substances: dithiocarb, ethanol and tetrahydrofurane. Increased release of hydrocarbons into the gas phase, after treatment with compounds suspected to induce lipid peroxidation, may result from decreased metabolism. If hydrocarbon exhalation was only moderately elevated as after ethanol administration, this aspect must be taken into account. Peroxidizing microsomes showed elevated hydrocarbon output when treated with CO in order to block metabolism. This study provided a procedure for discrimination of inhibitory and peroxidative action which was of importance if the increase over control levels was only moderate. It may serve as monitor for metabolic capacity of a laboratory animal.