CLONING OF THE MYCOBACTERIAL EPITOPE RECOGNIZED BY LYMPHOCYTES-T IN ADJUVANT ARTHRITIS
CLONING OF THE MYCOBACTERIAL EPITOPE RECOGNIZED BY LYMPHOCYTES-T IN ADJUVANT ARTHRITIS
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DOI：
10.1038/331171a0
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发表时间：
1988-01-14
期刊：
影响因子：
64.8
通讯作者：
COHEN, IR
中科院分区：
文献类型：
--
作者：
VANEDEN, W;THOLE, JER;COHEN, IR
佐剂性关节炎（AA）是一种慢性疾病诱导的大鼠免疫与抗原结核分枝杆菌1。在分离出致关节炎T细胞系2和克隆3后，有可能证明关键的M.结核抗原含有与关节软骨中自身抗原交叉反应的表位4 -6。与AA大鼠一样，类风湿性关节炎患者表现出对M的特异性T淋巴细胞反应性。含有交叉反应性表位的结核组分7.对临界M.我们用AA T细胞克隆筛选大肠杆菌表达的分枝杆菌抗原和基因工程截短的蛋白质和合成肽。AA T细胞克隆识别由牛分枝杆菌BCG抗原序列中180-188位氨基酸形成的表位8。给大鼠施用这种抗原诱导了对随后产生AA的尝试的抗性。
Adjuvant arthritis (AA) is a chronic disease inducible in rats by immunization with an antigen ofMycobacterium tuberculosis1. After the isolation of arthritogenic T-cell lines2and clones3, it became possible to demonstrate that the criticalM. tuberculosisantigen contained an epitope cross-reactive with a self-antigen in joint cartilage4–6. Like AA rats, patients suffering from rheumatoid arthritis demonstrated specific T-lymphocyte reactivity to theM. tuberculosisfraction containing the cross-reactive epitope7. To characterize the criticalM. tuberculosisepitope we used AA T-cell clones to screen mycobacterial antigens expressed inEscherichia coliand genetically engineered truncated proteins and synthetic peptides. The AA T-cell clones recognized an epitope formed by the amino acids at positions 180–188 in the sequence of aMycobacterium bovisBCG antigen8. Administration of this antigen to rats induced resistance to subsequent attempts to produce AA.