Enhanced Fumaric Acid Production by Fermentation of Xylose Using a Modified Strain of Rhizopus arrhizus
Enhanced Fumaric Acid Production by Fermentation of Xylose Using a Modified Strain of Rhizopus arrhizus
复制标题
DOI：
10.15376/biores.8.2.2186-2194
复制
发表时间：
2013-03
期刊：
影响因子：
1.5
通讯作者：
Shuang Wen;Luo Liu;Kaili Nie;Li Deng;T. Tan;Fang Wang
中科院分区：
文献类型：
--
作者：
Shuang Wen;Luo Liu;Kaili Nie;Li Deng;T. Tan;Fang Wang
通过增加木糖浓度的培养基经改良，成功地培育出一株木糖利用率较高的根霉菌株。木糖是一种相对便宜的单糖，可以很容易地从玉米芯、稻草和酒糟中获得，是种子培养阶段和发酵过程中使用的唯一碳源。经过改良，该菌株在利用木糖生产富马酸方面表现出了巨大的工业潜力。在初始木糖质量分数为8%、碳氮比为200、停留时间为7d的条件下，富马酸产量提高到28.48g/L(亲本为13.23g/L)。容积生产力为每小时169.52 mg/L(78.75 mg/L)。
An enhanced, xylose-utilizing strain of Rhizopus arrhizus was successfully developed via modification using media with increasing concentrations of xylose. Xylose, a relatively cheap monosugar that can be easily obtained from corncobs, rice straw, and vinasse, was the sole carbon source used for both the seed culture phase and the fermentation process. After modification, this newly improved strain showed tremendous industrial potential for fumaric acid production using xylose. The fumaric acid production increased to 28.48 g/L (parental strain was 13.23 g/L) at 8% initial xylose, a carbon:nitrogen ratio of 200, and a residence time of 7 days. The volumetric productivity was 169.52 mg/L (78.75 mg/L) per hour.