Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation
Inhibition of protein kinase D disrupts spindle formation and actin assembly during porcine oocyte maturation
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抑制蛋白激酶 D 会破坏猪卵母细胞成熟过程中纺锤体的形成和肌动蛋白的组装
DOI：
10.18632/aging.101667
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Sun Shao-Chen
中科院分区：
文献类型：
--
作者：
Zhang Yu;Wang Hong-Hui;Wan Xiang;Xu Yao;Pan Meng-Hao;Sun Shao-Chen
蛋白激酶D（PKD）亚家族包括PKD1、PKD2和PKD3，是一类新型的丝氨酸/苏氨酸激酶家族。PKD已被广泛证实参与多种生理效应的调控，包括免疫反应、细胞凋亡和细胞增殖 。（原句cell proliferat表述有误，推测应为cell proliferation）
Protein kinase D (PKD) subfamily which includes PKD1, PKD2 and PKD3 is a novel family of serine/threonine kinases. PKD has been widely implicated in the regulation of multiple physiological effects including immune responses, apoptosis and cell proliferation. However, the roles of PKD in oocytes have not been fully clarified. In this study we investigated the regulatory functions of PKD during porcine oocyte maturation. Our results indicated that PKD expressed in porcine oocytes and the inhibition of PKD family activity led to the failure of meiosis resumption and the first polar body extrusion. Further analysis indicated that the spindle assembly and chromosome alignment were disrupted after PKD family inhibition, and this might be through its regulatory role on MAPK phosphorylation. We also found that PKD phosphorylated cofilin for actin assembly, which further affected cortical actin distribution, indicating the roles of PKD family on cytoskeleton. In addition, a decreased expression of PKD in postovulatory aging porcine oocytes was observed, which might connect PKD with cytoskeleton defects in aged oocytes. Taken together, these results suggest that PKD possesses important functions in porcine oocyte maturation by regulating spindle organization and actin assembly.
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影响因子：
21.3
作者：
Eiseler T;Döppler H;Yan IK;Kitatani K;Mizuno K;Storz P
通讯作者：
Storz P
影响因子：
3.7
作者：
Tandon M;Salamoun JM;Carder EJ;Farber E;Xu S;Deng F;Tang H;Wipf P;Wang QJ
通讯作者：
Wang QJ
影响因子：
6.7
作者：
D. Navot;M. Drews;P. Bergh;I. Guzman;Alexis Karstaedt;R. Scott;G. Garrisi;G. Hofmann
通讯作者：
D. Navot;M. Drews;P. Bergh;I. Guzman;Alexis Karstaedt;R. Scott;G. Garrisi;G. Hofmann
影响因子：
16.6
作者：
Laband K;Le Borgne R;Edwards F;Stefanutti M;Canman JC;Verbavatz JM;Dumont J
通讯作者：
Dumont J
DOI：
10.1083/jcb.201606077
发表时间：
2017-11-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bury L;Coelho PA;Simeone A;Ferries S;Eyers CE;Eyers PA;Zernicka-Goetz M;Glover DM
通讯作者：
Glover DM