Localization and developmental expression of two chicken host defense peptides: cathelicidin-2 and avian β-defensin 9
Localization and developmental expression of two chicken host defense peptides: cathelicidin-2 and avian β-defensin 9
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DOI：
10.1016/j.dci.2016.03.008
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发表时间：
2016-08-01
影响因子：
2.9
通讯作者：
Haagsman, Henk P.
中科院分区：
文献类型：
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作者：
Cuperus, Tryntsje;van Dijk, Albert;Haagsman, Henk P.
在生命的最初几周，由于免疫系统的不成熟，小鸡对传染病非常敏感。在此期间，宿主防御肽（HDPs）的表达知之甚少。在这项研究中，我们研究了表达模式的两个鸡HDPs，cathelicidin CATH-2和β-防御素AvBD 9免疫组化在一组器官从胚胎第12天，直到4周posthatch。AvBD 9主要存在于整个肠道的肠内分泌细胞中，这是该细胞类型中体内HDP表达的首次报道，并且在发育期间显示稳定的表达水平。CATH-2仅存在于异嗜性细胞中，在大多数检查的器官包括脾脏、囊和小肠中，异嗜性细胞在孵化后减少。在肺组织中，CATH-2的表达呈双相性，并在孵化后第一天达到峰值。简而言之，CATH-2和AvBD 9似乎在战略定位以响应感染刺激的细胞类型中表达，表明这些肽在胚胎和早期孵化后防御中发挥作用。(C)2016爱思唯尔有限公司版权所有
In the first weeks of life young chickens are highly susceptible to infectious diseases due to immaturity of the immune system. Little is known about the expression of host defense peptides (HDPs) during this period. In this study we examined the expression pattern of two chicken HDPs, the cathelicidin CATH-2 and the beta-defensin AvBD9 by immunohistochemistry in a set of organs from embryonic day 12 until four weeks posthatch. AvBD9 was predominantly found in enteroendocrine cells throughout the intestine, the first report of in vivo HDP expression in this cell type, and showed stable expression levels during development. CATH-2 was exclusively found in heterophils which decreased after hatch in most of the examined organs including spleen, bursa and small intestine. In the lung CATH-2 expression was biphasic and peaked at the first day posthatch. In short, CATH-2 and AvBD9 appear to be expressed in cell types strategically located to respond to infectious stimuli, suggesting these peptides play a role in embryonic and early posthatch defense. (C) 2016 Elsevier Ltd. All rights reserved.