Screening mutations in drug-resistant Mycobacterium tuberculosis strains in Yunnan, China
Screening mutations in drug-resistant Mycobacterium tuberculosis strains in Yunnan, China
复制标题
中国云南省结核分枝杆菌耐药菌株突变筛选
DOI：
10.1016/j.jiph.2017.04.008
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发表时间：
2017-09-01
影响因子：
6.7
通讯作者：
Zhang, A-Mei
中科院分区：
文献类型：
--
作者：
Li, Daoqun;Song, Yuzhu;Zhang, A-Mei
耐药结核病(DR-TB)，尤其是耐多药结核病(MDR-TB)是中国面临的严重医疗和社会问题。本研究旨在探讨云南地区结核分枝杆菌(简称结核分枝杆菌)中国耐药株的突变特征。对523株临床分离的结核分枝杆菌进行药敏试验。选择6个耐药基因(katG、inha、rpoB、rpsL、embB和pncA)进行突变筛查。DST共鉴定出54株临床结核分枝杆菌耐药株，其中18株为单一耐药株，36株为多药耐药株。在41个菌株中进行了5个基因(不包括inha基因)的24种类型的突变筛查。在这项研究中发现了6个新的突变，包括3个错义突变(katG中的p.S302R，embB中的p.D78G，以及pncA中的p.M1I)，两个分支移位突变(408个Ins A和538-580 del在pncA中)，以及一个控制区的突变(-6C>；T位于rps L的上游)。KatG、rpoB、rpsL、embB和pncA基因热点突变区的突变频率分别为92.5%、44.4%、54.2%、52.6%和37.5%。突变谱和突变频率似乎在云南DR-TB株中是独一无二的。(C)2017年作者。由Elsevier Limited代表沙特国王本·阿卜杜勒阿齐兹健康科学大学出版。
Drug-resistant tuberculosis (DR-TB), especially multidrug-resistant tuberculosis (MDR-TB), is a seriousmedical and societal problem in China. The purpose of this study was to evaluate the mutation characteristics of drug-resistant Mycobacterium tuberculosis (M. tuberculosis) isolates in Yunnan, China. Drug susceptibility testing (DST) was performed in 523 clinical M. tuberculosis isolates. Six drug resistancegenes (katG, inhA, rpoB, rpsL, embB, and pncA) were selected to screen for mutations. In total, 54 clinical M. tuberculosis strains were identified as drug-resistant by DST, including 18 single drug-resistant( SDR) strains and 36 multidrug-resistant (MDR) strains. Twenty-four types of mutations in five genes( excluding the inhA gene) were screened in forty-one strains. Six novel mutations were identified in thisstudy, including three missense mutations (p. S302R in katG, p. D78G in embB, and p. M1I in pncA), twoframeshift mutations (408 ins A and 538-580 del in pncA), and one mutation in a control region (-6 C>T located upstream of rpsL). The mutation frequencies in the hotspot mutation regions in the katG, rpoB, rpsL, embB, and pncA genes were 92.5%, 44.4%, 54.2%, 52.6%, and 37.5%, respectively. The mutation spectraand frequencies seemed some what unique in the Yunnan DR-TB strains. (C) 2017 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences.