Fructanase and fructosyltransferase activity of non-Saccharomyces yeasts isolated from fermenting musts of Mezcal
Fructanase and fructosyltransferase activity of non-Saccharomyces yeasts isolated from fermenting musts of Mezcal
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DOI：
10.1016/j.biortech.2012.01.112
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发表时间：
2012-04-01
影响因子：
11.4
通讯作者：
Monsan, Pierre
中科院分区：
文献类型：
--
作者：
Arrizon, Javier;Morel, Sandrine;Monsan, Pierre
果糖酶和果糖基转移酶是龙舌兰酒加工和益生元生产（功能食品工业）的重要组成部分。本研究对从“Mezcal de Oaxaca”中分离的130株非酵母菌进行了果糖酶和果糖基转移酶活性的筛选。在固体培养基上，50株分离株分别在龙舌兰（Agave requilana fructans， ATF）、菊粉和levan上生长。在液体培养基中，菊粉和ATF诱导的果糖酶活性在0.02 ~ 0.27 U/ml之间，具体取决于酵母菌分离物。对蔗糖的果糖酶活性最高的是马氏克鲁维菌和德尔布鲁氏环孢菌，而对菊粉和ATF的果糖酶活性最高的是东方伊萨琴菌、白隐球菌和尖假丝酵母。双孢酵母和假丝酵母对酵母菌有较高的水解活性。马氏酵母、delbrueckii和顶孢酵母的16株酵母果糖转移酶活性均为阳性。Mezcal菌群被证明是新型果糖酶和果糖基转移酶的来源，在龙舌兰酒和食品工业中具有潜在的应用前景。(C) 2012 Elsevier Ltd.版权所有。
Fructanase and fructosyltransferase are interesting for the tequila process and prebiotics production (functional food industry). In this study, one hundred thirty non-Saccharomyces yeasts isolated from "Mezcal de Oaxaca" were screened for fructanase and fructosyltransferase activity. On solid medium, fifty isolates grew on Agave requilana fructans (ATF), inulin or levan. In liquid media, inulin and ATF induced fructanase activities of between 0.02 and 0.27 U/ml depending of yeast isolate. High fructanase activity on sucrose was observed for Kluyveromyces marxianus and Torulaspora delbrueckii, while the highest fructanase activity on inulin and ATF was observed for Issatchenkia orientalis, Cryptococcus albidus, and Candida apicola. Zygosaccharomyces bisporus and Candida boidinii had a high hydrolytic activity on levan. Sixteen yeasts belonging to K. marxianus, T. delbrueckii and C. apicola species were positive for fructosyltransferase activity. Mezcal microbiota proved to showed to be a source for new fructanase and fructosyltransferases with potential application in the tequila and food industry. (C) 2012 Elsevier Ltd. All rights reserved.