Spongiibacter marinus gen. nov., sp nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp 1
Spongiibacter marinus gen. nov., sp nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp 1
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DOI：
10.1099/ijs.0.65438-0
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发表时间：
2008-03-01
影响因子：
2.8
通讯作者：
Szewzyk, Ulrich
中科院分区：
文献类型：
--
作者：
Graeber, Ingeborg;Kaesler, Ines;Szewzyk, Ulrich
菌株HAL 40 b（T）是从采自挪威海岸外苏拉海岭的海绵Haliclona sp. 1中分离到的，并对其进行了生理生化和系统发育分析。该分离物为具有极鞭毛的小杆状物。它是好氧的，革兰氏阴性，氧化酶和过氧化氢酶阳性。在20-30 ℃、pH 7-9和3%NaCl中观察到最佳生长。底物利用试验对阿拉伯糖、吐温40和吐温80呈阳性。酶试验阳性碱性磷酸酶，酯酶脂肪酶（CB），亮氨酸芳基酰胺酶，酸性磷酸酶，萘酚-AS-BI-磷酸水解酶和N-乙酰-β-氨基葡萄糖苷酶。主要的细胞脂肪酸是C-17：1 omega 8，其次是C-17：0和C-18：1 omega 7。基质辅助激光解吸/电离飞行时间MS分析用于表征菌株，产生特征性的低分子量蛋白质模式，可用作该物种成员的识别指纹。DNA G+C含量为69.1mol%。16 S rRNA基因序列比较支持的系统发育分析将该菌株分类为γ-变形菌纲的成员。菌株HAL 40 b（T）与其它海洋细菌Neptunomonasnaphthovorans和Marinaproensis（模式菌株序列相似性>90%）的亲缘关系较远。根据该菌株的表型、生理特性和系统发育特征，建议将其作为一个新种的代表，归入一个新属，即海海绵杆菌（Spongiibacter marinus gen. nov.），sp. nov.;海绵状杆菌的模式菌株是HAL 40 b（T）（=DSM 17750（T）=CCUG 54896（T））。
Strain HAL40b(T) was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optimal growth was observed at 20-30 degrees C, pH 7-9 and in 3% NaCl. Substrate utilization tests were positive for arabinose, Tween 40 and Tween 80. Enzyme tests were positive for alkaline phosphatase, esterase lipase (CB), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-beta-glucosaminidase. The predominant cellular fatty acid was C-17: 1 omega 8, followed by C-17: 0 and C-18: 1 omega 7. Analysis by matrix-assisted laser desorption/ionization time-of-flight MS was used to characterize the strain, producing a characteristic low-molecular-mass protein pattern that could be used as a fingerprint for identification of members of this species. The DNA G+C content was 69.1 mol%. Phylogenetic analysis supported by 16S rRNA gene sequence comparison classified the strain as a member of the class Gammaproteobacteria. Strain HAL40b(T) was only distantly related to other marine bacteria including Neptunomonas naphthovorans and Marinobacter daepoensis (type strain sequence similarity >90%). Based on its phenotypic, physiological and phylogenetic characteristics, it is proposed that the strain should be placed into a new genus as a representative of a novel species, Spongiibacter marinus gen. nov., sp. nov.; the type strain of Spongiibacter marinus is HAL40b(T) (=DSM 17750(T) =CCUG 54896(T)).