The pathogenicity of Vairimorpha necatrix (Microspora: Microsporidia) against the tomato moth, Lacanobia oleracea (Lepidoptera:Noctuidae) and its potential use for the control of lepidopteran glasshouse pests
The pathogenicity of Vairimorpha necatrix (Microspora: Microsporidia) against the tomato moth, Lacanobia oleracea (Lepidoptera:Noctuidae) and its potential use for the control of lepidopteran glasshouse pests
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DOI：
10.1002/ps.872
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发表时间：
2004-08-01
影响因子：
4.1
通讯作者：
Edwards, JP
中科院分区：
文献类型：
--
作者：
Down, RE;Bell, HA;Edwards, JP
采用液滴饲喂技术，将已知量的Vairimorpha necatrix（克雷默）孢子饲喂番茄蛾Lacanobia oleracea（L）幼虫，以评估该鳞翅目害虫对病原体的敏感性。所有的第一至第四龄幼虫死亡的结果，ingrivent1000或更多的V necatrix孢子。观察到两种形式的死亡，这取决于剂量和处理时昆虫的年龄。对于一龄幼虫，在摄入2000个孢子后发生快速死亡（给药后6天内），而较低剂量导致部分幼虫死于慢性感染（微孢子虫病）。对于更高级的阶段，需要增加孢子剂量以快速死亡，例如需要200 000个孢子的剂量才能在6天内使三龄幼虫死亡率达到80%。在四至六龄幼虫中未观察到快速死亡。在所有情况下，成功的化蛹和成人出现大大减少相比，非感染laviers.V necatestrium的悬浮液喷洒到番茄（番茄工厂）保持在小温室中的植物，L oleracea幼虫侵染之前。从喷洒V necatrix的植物中回收的害虫幼虫的数量和生物量分别显著降低了40%和70%，与用水喷洒的植物（对照）相比。同样，与对照植物相比，喷洒V necatrix的植物显示出高达45%的损害减少。版权所有2004年。经英国政府文书局许可复制。出版社：John Wiley Sons，Ltd.
A droplet feeding technique was used to feed known amounts of Vairimorpha necatrix (Kramer) spores to larvae of the tomato moth, Lacanobia oleracea (L) in order to assess the susceptibility of this lepidopteran pest to the pathogen. All first- to fourth-instar larvae died as a result of ingesting 1000 or more V necatrix spores. Two forms of death were observed, which were dependent on the dose and the age of the insect when treated. For first-instar larvae, rapid death (within 6 days of dosing) occurred after ingestion of 2000 spores, whereas lower doses resulted in a proportion of larvae dying from chronic infection (microsporidiosis). For more advanced stages, increasing spore doses were required to give rapid death, such that a dose of 200 000 spores was needed to give 80% mortality within 6 days for third-instar larvae. Rapid death was not observed in fourth- to sixth-instar larvae. In all cases successful pupation and adult emergence were much reduced compared with non-infected larvae.Suspensions of V necatvix were sprayed on to tomato (Lycopersicon esculentum Mill) plants maintained in small glasshouses prior to infestation of the plants with L oleracea larvae. The numbers and biomass of pest larvae retrieved from the plants sprayed with V necatrix were significantly reduced by up to 40% and 70%, respectively, compared with plants sprayed with water (control). Similarly, plants sprayed with V necatrix showed a reduction in damage of up to 45% compared with the control plants. British Crown copyright 2004. Reproduced with the permission of Her Majesty's Stationery Office. Published for SCI by John Wiley Sons, Ltd.