Functional Vanilloid Receptor-1 in Human Submandibular Glands
Functional Vanilloid Receptor-1 in Human Submandibular Glands
复制标题
人颌下腺中的功能性香草酸受体 1
DOI：
10.1177/0022034510366841
复制
发表时间：
2010-07-01
影响因子：
7.6
通讯作者：
Yu, G. Y.
中科院分区：
文献类型：
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作者：
Ding, Q. W.;Zhang, Y.;Yu, G. Y.
香草酸受体-1(VR1)最早发现于神经系统。最近的证据表明，VR1也在各种类型的细胞中表达。我们假设VR1存在于人的颌下腺(SMG)中，并参与调节唾液分泌。VR1mRNA和蛋白在人唾液间质管上皮细胞系和人唾液腺间质管细胞系中均有表达。VR1主要定位于浆液性腺泡细胞和导管细胞，粘液性腺泡细胞不表达。辣椒素是VR1的激动剂，它能增加细胞内游离钙离子浓度，促进细胞外信号调节激酶的磷酸化，并诱导水通道蛋白5(AQP5)从细胞质向质膜转运。这些作用可被VR1拮抗剂卡萨西平预先处理而取消。此外，将辣椒素霜涂在覆盖颌下区的皮肤上，可以增加唾液分泌。这些发现表明，在人类SMG中表达有功能的VR1，并通过调节AQP5的运输参与调节唾液分泌。
Vanilloid receptor-1 (VR1) was originally found in the nervous system. Recent evidence indicates that VR1 is also expressed in various cell types. We hypothesized that VR1 exists in the human submandibular gland (SMG) and is involved in regulating salivary secretion. VR1 mRNA and protein were expressed in human SMGs and a human salivary intercalated duct cell line. VR1 was mainly located in serous acinar and ductal cells, but not in mucous acinar cells. Capsaicin, an agonist of VR1, increased intracellular free calcium, enhanced phosphorylation of extracellular signal-regulated kinase, and induced the trafficking of aquaporin 5 (AQP5) from the cytoplasm to the plasma membrane. These effects were abolished by pre-treatment with the VR1 antagonist capsazepine. Furthermore, capsaicin cream applied to the skin covering the submandibular area increased salivary secretion. These findings indicated that a functional VR1 is expressed in the human SMG and is involved in regulating salivary secretion by mediating AQP5 trafficking.