Nanoceria Aggregate Formulation Promotes Buffer Stability, Cell Clustering, and Reduction of Adherent Biofilm in Streptococcus mutans.
Nanoceria Aggregate Formulation Promotes Buffer Stability, Cell Clustering, and Reduction of Adherent Biofilm in Streptococcus mutans.
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DOI：
10.1021/acsbiomaterials.3c00174
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发表时间：
2023-07
影响因子：
5.8
通讯作者：
K. Ellepola;L. Bhatt;Lin Chen;Chen Han;Forough Jahanbazi;R. Klie;Francisco J. Lagunas Vargas;Yuanbing Mao;Kirill Novakovsky;Bibash Sapkota;R. Pesavento
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文献类型：
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作者：
K. Ellepola;L. Bhatt;Lin Chen;Chen Han;Forough Jahanbazi;R. Klie;Francisco J. Lagunas Vargas;Yuanbing Mao;Kirill Novakovsky;Bibash Sapkota;R. Pesavento
变形链球菌是牙源性生物膜发育的关键病因之一，在可发酵糖存在的情况下导致龋齿。我们之前报道过铈盐水解产生的酸稳定纳米铈（CeO2-NP）通过非杀菌机制限制变形链球菌粘附生物膜的能力。在此，我们报道了一种硫酸软骨素a （CSA）配方（CeO2-NP-CSA），该配方包含纳米粒聚集体，可在一系列条件下促进对大量沉淀的抵抗力，同时保留生物膜抑制活性，从而允许对其生物活性进行更彻底的机制研究。CeO2-NP-CSA降低变形链球菌体外生物膜粘附性的主要机制是产生非粘附细胞团。此外，剂量依赖的体外人体细胞毒性研究表明，除了目前用于许多口腔保健产品的等摩尔剂量的氟化钠外，没有其他毒性。这项研究代表了一种独特的方法和使用纳米粒聚集体配方，具有促进口腔健康和预防龋齿作为辅助治疗的意义。
Streptococcus mutans is one of the key etiological factors in tooth-borne biofilm development that leads to dental caries in the presence of fermentable sugars. We previously reported on the ability of acid-stabilized nanoceria (CeO2-NP) produced by the hydrolysis of ceric salts to limit biofilm adherence of S. mutans via non-bactericidal mechanism(s). Herein, we report a chondroitin sulfate A (CSA) formulation (CeO2-NP-CSA) comprising nanoceria aggregates that promotes resistance to bulk precipitation under a range of conditions with retention of the biofilm-inhibiting activity, allowing for a more thorough mechanistic study of its bioactivity. The principal mechanism of reduced in vitro biofilm adherence of S. mutans by CeO2-NP-CSA is the production of nonadherent cell clusters. Additionally, dose-dependent in vitro human cell toxicity studies demonstrated no additional toxicity beyond that of equimolar doses of sodium fluoride, currently utilized in many oral health products. This study represents a unique approach and use of a nanoceria aggregate formulation with implications for promoting oral health and dental caries prevention as an adjunctive treatment.