Rapid signal transduction in living cells is a unique feature of mechanotransduction
Rapid signal transduction in living cells is a unique feature of mechanotransduction
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DOI：
10.1073/pnas.0711704105
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发表时间：
2008-05-06
影响因子：
11.1
通讯作者：
Wang, Ning
中科院分区：
文献类型：
--
作者：
Na, Sungsoo;Collin, Olivier;Wang, Ning
人们普遍假定，机械力转导是通过诱导蛋白质的局部构象变化在局部力 - 膜界面处启动的，这类似于可溶性配体诱导的信号转导。然而，所有已发表的报告在时间尺度上都存在局限性，无法解决这一基本问题。在活细胞中使用基于荧光共振能量转移（FRET）的胞质Src报告基因，我们对通过激活的整合素施加局部应力时Src活性的变化进行了量化。该应力诱导了快速的（
（最后一个单词“rapid”后面似乎内容不完整，如果还有遗漏的部分，请告诉我，以便我更好地为你翻译。）
It is widely postulated that mechanotransduction is initiated at the local force-membrane interface by inducing local conformational changes of proteins, similar to soluble ligand-induced signal transduction. However, all published reports are limited in time scale to address this fundamental issue. Using a FRET-based cytosolic Src reporter in a living cell, we quantified changes of Src activities as a local stress via activated integrins was applied. The stress induced rapid (