Simultaneous measurements of lymphatic vessel contraction, flow and valve dynamics in multiple lymphangions using optical coherence tomography
Simultaneous measurements of lymphatic vessel contraction, flow and valve dynamics in multiple lymphangions using optical coherence tomography
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DOI：
10.1002/jbio.201700017
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发表时间：
2018-08-01
影响因子：
2.8
通讯作者：
Vakoc, Benjamin J.
中科院分区：
文献类型：
--
作者：
Blatter, Cedric;Meijer, Eelco F. J.;Vakoc, Benjamin J.
淋巴功能障碍涉及许多疾病，包括淋巴水肿、高血压、自身免疫反应、移植物排斥、动脉粥样硬化、微生物感染、癌症和癌症转移。扩大我们对淋巴系统功能的了解可以更好地了解这些疾病的过程，并改善治疗方案。本研究采用光学相干断层扫描（OCT）方法三维显示腔内瓣膜动力学，同时测量小鼠腘窝淋巴结的传入集淋巴管的3个邻近淋巴管的淋巴流动和血管收缩。血流测量是基于多普勒OCT技术结合外源性淋巴标记的Intralipid。通过这些成像方法，可以更全面地研究淋巴功能和泵送。这些能力可以使我们更好地理解健康和疾病中淋巴管的调节和失调。该图像描述了用光学相干断层扫描在体内同时测量的淋巴阀、淋巴管横截面积和淋巴速度的动态测量。
Lymphatic dysfunction is involved in many diseases including lymphedema, hypertension, autoimmune responses, graft rejection, atherosclerosis, microbial infections, cancer and cancer metastasis. Expanding our knowledge of lymphatic system function can lead to a better understanding of these disease processes and improve treatment options. Here, optical coherence tomography (OCT) methods were used to reveal intraluminal valve dynamics in 3 dimensions, and measure lymph flow and vessel contraction simultaneously in 3 neighboring lymphangions of the afferent collecting lymphatic vessels to the popliteal lymph node in mice. Flow measurements were based on Doppler OCT techniques in combination with exogenous lymph labeling by Intralipid. Through these imaging methods, it is possible to study lymphatic function and pumping more comprehensively. These capabilities can lead to a better understanding of the regulation and dysregulation of lymphatic vessels in health and disease. The image depicts the dynamic measurements of lymphatic valves, lymphatic vessels cross-sectional area and lymph velocity simultaneously measured in vivo with optical coherence tomography.