Cryopreservation affects ROS-induced oxidative stress and antioxidant response in Arabidopsis seedlings
Cryopreservation affects ROS-induced oxidative stress and antioxidant response in Arabidopsis seedlings
复制标题
低温保存影响拟南芥幼苗中ROS诱导的氧化应激和抗氧化反应。
DOI：
10.1016/j.cryobiol.2014.11.004
复制
发表时间：
2015-02-01
期刊：
影响因子：
2.7
通讯作者：
Shen, Xiao-hui
中科院分区：
文献类型：
--
作者：
Chen, Guan-qun;Ren, Li;Shen, Xiao-hui
玻璃化法超低温保存后植株的恢复状况与活性氧诱导的氧化胁迫呈负相关。以萌发48 h和72 h的拟南芥幼苗为材料，研究了超低温保存5个阶段中ROS（H2 O2和OW）和抗氧化系统（SOD、POD、CAT、阿萨和GSH）在低温伤害中的作用。此外，以丙二醛（MDA）为指标，研究了各处理对膜脂过氧化的影响。结果表明，H2 O2诱导的氧化胁迫是导致48 h幼苗（高成活率）和72 h幼苗（无成活率）低温伤害的主要原因。Amplex Red染色结果表明，H_2O_2主要产生于子叶、茎尖和根中。72小时的幼苗存活率低，与严重的膜脂过氧化，这是由增加OH。降低了SOD活性。qRT-PCR和生理生化分析表明，48 h幼苗的抗氧化系统被活性氧激活，并对氧化胁迫进行防御。高水平的ROS导致72 h幼苗抗氧化系统的减弱。相关性分析表明，抗氧化酶活性的增强是48 h幼苗存活率高的原因，这可以通过抗氧化突变体幼苗的超低温保存来反映。该模型表明，CAT活性和阿萨含量的升高是决定低温胁迫耐受性的重要因素，对它们的调控可以提高低温保存后幼苗的恢复率。(C)2014爱思唯尔公司All rights reserved.
Plant recovery status after cryopreservation by vitrification had a negative relationship to the oxidative stress induced by reactive oxygen species (ROS). Arabidopsis thaliana seedlings germinated for 48 h or 72 h with different survival tolerances were examined at five steps of cryopreservation, to determine the role of ROS H2O2 and OW) and antioxidant systems (SOD, POD, CAT, AsA and GSH) in cryo-injury. In addition, the effects of the steps on membrane lipid peroxidation were studied using malondialdehyde (MDA) as an indicator. The results indicated that H2O2-induced oxidative stress at the steps of dehydration and rapid warming was the main cause of cryo-injury of 48-h seedlings (high survival rate) and 72-h seedlings (no survival). The H2O2 was mainly generated in cotyledons, shoot tips and roots of seedlings as indicated by Amplex Red staining. Low survival of 72-h seedlings was associated with severe membrane lipid peroxidation, which was caused by increased OH. generation activity and decreased SOD activity. The antioxidant-related gene expression by qRT-PCR and physiological assays suggested that the antioxidant system of 48-h seedlings were activated by ROS, and they mounted a defense against oxidative stress. A high level of ROS led to the Weakening of the antioxidant system of 72-h seedlings. Correlation analysis indicated that enhanced antioxidant enzymes activities contributed to the high survival rate of 48-h seedlings, which could reflect by cryopreservation of antioxidant mutant seedlings. This model system indicated that elevated CAT activity and AsA content were determinants of cryogenic stress tolerance, whose manipulation could improve the recovery of seedlings after cryopreservation. (C) 2014 Elsevier Inc. All rights reserved.