INVIVO IMAGING OF MONOAMINERGIC NERVE-TERMINALS IN NORMAL AND MPTP-LESIONED PRIMATE BRAIN USING POSITRON EMISSION TOMOGRAPHY (PET) AND [C-11] TETRABENAZINE
INVIVO IMAGING OF MONOAMINERGIC NERVE-TERMINALS IN NORMAL AND MPTP-LESIONED PRIMATE BRAIN USING POSITRON EMISSION TOMOGRAPHY (PET) AND [C-11] TETRABENAZINE
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DOI：
10.1002/syn.890140205
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发表时间：
1993-06-01
期刊：
影响因子：
2.3
通讯作者：
DOMINO, EF
中科院分区：
文献类型：
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作者：
DASILVA, JN;KILBOURN, MR;DOMINO, EF
利用[C-11]四苯嗪([C-11]TBZ)和正电子发射断层扫描(PET)技术，首次在活体灵长类动物脑内成功地进行了单胺类囊泡转运体的成像。放射性配基进入大脑的速度很快，在较短的时间内(10-30分钟)，多巴胺能神经支配较密集的纹状体可以清楚地看到放射性示踪剂的摄取和滞留。联合注射药理剂量(1 mg/kg，i.v)可完全阻断纹状体的特异性结合。四苯那嗪。在单侧MPTP损毁猴，损毁侧纹状体缺乏放射性配基的特异性结合，对皮质、小脑或对侧纹状体的放射性示踪剂分布无影响。[C-11]TBZ的PET成像为在体研究单胺能神经元及其在神经退行性疾病中的丢失提供了新的途径。
The first successful in vivo imaging of monoamine vesicular transporters in the living primate brain is described, using [C-11]tetrabenazine ([C-11]TBZ) and Positron Emission Tomography (PET). Radioligand uptake into brain is rapid, and at short time periods (10-30 minutes) the higher uptake and retention of the radiotracer in the more densely dopaminergic innervated striatum is clearly visualized. Specific binding in striatum can be entirely blocked with co-administration of a pharmacological dose (1 mg/kg i.v.) of tetrabenazine. In a unilaterally MPTP-lesioned monkey, specific binding of radioligand was absent in the, striatum on the lesioned side, with no effect on radiotracer distribution in the cortex, cerebellum or contralateral striatum. PET imaging with [C-11]TBZ provides a new approach to the in vivo study of monoaminergic neurons and their loss in neurodegenerative diseases.