Silver percutaneous absorption after exposure to silver nanoparticles: A comparison study of three human skin graft samples used for clinical applications
Silver percutaneous absorption after exposure to silver nanoparticles: A comparison study of three human skin graft samples used for clinical applications
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DOI：
10.1016/j.burns.2014.02.003
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发表时间：
2014-11-01
期刊：
影响因子：
2.7
通讯作者：
Maina, G.
中科院分区：
文献类型：
--
作者：
Bianco, C.;Adami, G.;Maina, G.
银纳米颗粒（AgNPs）越来越多地应用于广泛的生物医学用途的材料。由于大量释放的银离子具有广谱的抗菌活性，因此能够与人体皮肤密切接触。银可以渗透皮肤;然而，没有关于银通过烧伤恢复中常用的皮肤移植物渗透的数据。我们研究的目的是使用Franz扩散池装置在暴露于AgNP在合成汗液中的悬浮液24小时后，使用新鲜的、冷冻保存的和甘油化的人皮肤移植物来评估银渗透。银渗透曲线显示与新鲜和冷冻保存的皮肤相比，通过甘油化皮肤的渗透显著更高：24小时银通量渗透为0.2 ng cm（-2）h（-1）（滞后时间：8.2小时）新鲜皮肤，0.3 ng cm（-2）h（-1）（滞后时间：10.9 h），3.8 ng cm（-2）h（-1）与新鲜和冷冻保存的皮肤相比，通过甘油化皮肤的渗透显著更高。这一结果可以产生相关的临床意义烧伤治疗的产品含有银纳米粒子。(C)2014 Elsevier Ltd和ISBI。All rights reserved.
Silver nanoparticles (AgNPs) are increasingly applied to a wide range of materials for biomedical use. These enable a close contact with human skin, thanks to the large release of silver ions that is responsible for a broad spectrum of antimicrobial activity. Silver can permeate the skin; however, there are no data available on silver permeation through skin grafts commonly used in burns recovery. The aim of our study was to evaluate silver penetration using fresh, cryopreserved, and glycerolized human skin grafts after exposure to a suspension of AgNPs in synthetic sweat using a Franz diffusion cell apparatus for 24 h.Silver permeation profiles revealed a significantly higher permeation through glycerolized skin compared with both fresh and cryopreserved skin: 24-h silver flux penetration was 0.2 ng cm(-2)h(-1) (lag time: 8.2 h) for fresh skin, 0.3 ng cm(-2)h(-1) (lag time: 10.9 h) for cryopreserved skin, and 3.8 ng cm(-2)h(-1) (lag time: 6.3 h) for glycerolized skin.Permeation through glycerolized skin is significantly higher compared to both fresh and cryopreserved skin. This result can generate relevant clinical implications for burns treatment with products containing AgNPs. (C) 2014 Elsevier Ltd and ISBI. All rights reserved.