Sulfur-doped carbon dots@polydopamine-functionalized magnetic silver nanocubes for dual-modality detection of norovirus
Sulfur-doped carbon dots@polydopamine-functionalized magnetic silver nanocubes for dual-modality detection of norovirus
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DOI：
10.1016/j.bios.2021.113540
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发表时间：
2021-08-14
影响因子：
12.6
通讯作者：
Park, Enoch Y.
中科院分区：
文献类型：
--
作者：
Achadu, Ojodomo J.;Abe, Fuyuki;Park, Enoch Y.
协同双模光学平台是传染病诊断和管理中的新兴检测工具。在这里，新的双模态荧光（FL）和表面增强拉曼散射（Sers）技术已被集成到一个单一的探针快速和超灵敏检测诺如病毒（NoV）。所开发的基于FL-SER的生物传感器依赖于新合成的硫掺杂琼脂衍生碳点（S-agCD）的双信号增强。抗原-抗体免疫反应导致在抗NoV抗体缀合的S-agCD和聚多巴胺官能化的磁性银纳米立方体[聚（dop）-MNPs-Ag NC]之间形成核心-卫星免疫复合物。通过部署免疫磁性富集方案并在单层石墨烯基底上进行Sers模态，在1 fg mL（-1）- 10 ng mL（-1）的宽范围内检测到诺如病毒样颗粒（NoV-LP），检测限为0.1 fg mL（-1）。生物传感器的双重信号传导特性的组合优势在对粪便样本中的临床NoV进行Sers检测之前使用FL共聚焦成像进行“热点”跟踪来证明，所述粪便样本低至约10个RNA拷贝mL（约1）。所提出的双模态生物传感器的性能增加了用于公共卫生的快速和低成本敏感的NoV检测和监测选项的前景。
Synergistic dual-mode optical platforms are up-and-coming detection tools in the diagnosis and management of infectious diseases. Here, novel dual-modality fluorescence (FL) and surface-enhanced Raman scattering (SERS) techniques have been integrated into a single probe for the rapid and ultrasensitive detection of norovirus (NoV). The developed FL-SER-based biosensor relies on the dual-signal enhancements of newly synthesized sulfur-doped agar-derived carbon dots (S-agCDs). The antigen-antibody immunoreaction results in forming a core-satellite immunocomplex between anti-NoV antibody-conjugated S-agCDs and polydopamine-functionalized magnetic silver nanocubes [poly (dop)-MNPs-Ag NCs]. By deploying an immunomagnetic enrichment protocol and performing the SERS modality on a single-layer graphene substrate, norovirus-like particles (NoV-LPs) were detected across a wide range of 1 fg mL(-1) - 10 ng mL(-1) with an excellent limit of detection of 0.1 fg mL(-1). The combined advantage of the dual-signaling properties of the biosensor was demonstrated using FL confocal imaging for "hotspots" tracking prior to SERS detection of clinical NoV in fecal specimen down to -10 RNA copies mL(-1). The proposed dual-modality biosensor's performance increases the prospect of a rapid and low-cost sensitive NoV detection and surveillance option for public health.Y