Temporally and Spatially Distinct Thirst Satiation Signals
Temporally and Spatially Distinct Thirst Satiation Signals
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DOI：
10.1016/j.neuron.2019.04.039
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发表时间：
2019-07-17
期刊：
影响因子：
16.2
通讯作者：
Oka, Yuki
中科院分区：
文献类型：
--
作者：
Augustine, Vineet;Ebisu, Haruka;Oka, Yuki
对于口渴的动物来说，摄入液体既能提供饱腹感，又能带来饮酒的乐趣。大脑是如何处理这些因素的，目前尚不清楚。在这里，我们确定了口渴满足的潜在神经回路，并检查了它们对奖励信号的贡献。我们发现，口渴驱动的神经元通过吞咽液体诱导的口咽刺激和肠道渗透压感觉，接收到时间上不同的饱足信号。我们证明，个体的口渴满足信号是由终板中不同的解剖学上独特的抑制性神经回路所传递的。此外，我们使用了一个超快的多巴胺(DA)传感器来检测口渴满足本身是否会刺激与奖励相关的回路。有趣的是，自发的饮酒行为，而不是减少口渴，引发了DA的释放。重要的是，在水分受限的条件下，对满足口渴的神经元的化学遗传刺激不会激活DA神经元。总而言之，这项研究剖析了口渴满足回路，其活动在功能上可与奖赏相关的大脑活动分开。
For thirsty animals, fluid intake provides both satiation and pleasure of drinking. How the brain processes these factors is currently unknown. Here, we identified neural circuits underlying thirst satiation and examined their contribution to reward signals. We show that thirst-driving neurons receive temporally distinct satiation signals by liquid-gulping-induced oropharyngeal stimuli and gut osmolality sensing. We demonstrate that individual thirst satiation signals are mediated by anatomically distinct inhibitory neural circuits in the lamina terminalis. Moreover, we used an ultrafast dopamine (DA) sensor to examine whether thirst satiation itself stimulates the reward-related circuits. Interestingly, spontaneous drinking behavior but not thirst drive reduction triggered DA release. Importantly, chemo-genetic stimulation of thirst satiation neurons did not activate DA neurons under water-restricted conditions. Together, this study dissected the thirst satiation circuit, the activity of which is functionally separable from reward-related brain activity.