A single-stranded architecture for cotranscriptional folding of RNA nanostructures
A single-stranded architecture for cotranscriptional folding of RNA nanostructures
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DOI：
10.1126/science.1253920
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发表时间：
2014-08-15
期刊：
影响因子：
56.9
通讯作者：
Andersen, Ebbe S.
中科院分区：
文献类型：
--
作者：
Geary, Cody;Rothemund, Paul W. K.;Andersen, Ebbe S.
人工DNA和RNA结构已被用作各种纳米级器件的支架。与DNA结构相比，RNA结构的大小有限，但它们也有优势：RNA可以在转录过程中折叠，因此可以在细胞中进行遗传编码和表达。我们介绍了一种架构，用于设计人工RNA结构，折叠从一条单链，其中阵列的反平行RNA螺旋精确组织的RNA三级基序和一种新型的交叉模式。我们构建了组装成六边形晶格的RNA瓦片，并证明了晶格可以通过退火和/或共转录折叠来形成。瓦片的长度可以扩大到660个核苷酸，达到与大型天然核酶相当的大小。
Artificial DNA and RNA structures have been used as scaffolds for a variety of nanoscale devices. In comparison to DNA structures, RNA structures have been limited in size, but they also have advantages: RNA can fold during transcription and thus can be genetically encoded and expressed in cells. We introduce an architecture for designing artificial RNA structures that fold from a single strand, in which arrays of antiparallel RNA helices are precisely organized by RNA tertiary motifs and a new type of crossover pattern. We constructed RNA tiles that assemble into hexagonal lattices and demonstrated that lattices can be made by annealing and/or cotranscriptional folding. Tiles can be scaled up to 660 nucleotides in length, reaching a size comparable to that of large natural ribozymes.