Hormone receptor levels and hormone, RNA and protein metabolism in the genital tract during the oestrous cycle in the ewe.
Hormone receptor levels and hormone, RNA and protein metabolism in the genital tract during the oestrous cycle in the ewe.
复制标题
母羊发情周期期间生殖道的激素受体水平以及激素、RNA 和蛋白质代谢。
DOI：
10.1677/joe.0.0730091
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发表时间：
1977
期刊：
影响因子：
2.8
通讯作者：
B. G. Miller
中科院分区：
文献类型：
--
作者：
G. Stone;L. Murphy;B. G. Miller
在发情周期的第0天（发情期）、第2天、第5天、第10天或第14天处死母羊，测定其生殖道中可溶性雌二醇和孕酮受体蛋白的浓度以及几种代谢活性。在泪阜子宫内膜和整个子宫中，雌二醇受体的浓度（pmol类固醇结合/mg组织DNA）在发情期最高，此后稳定下降，在第14天降至最低值。孕酮受体浓度在第0-5天最高，然后在第10-14天降至低水平。在整个子宫的切碎物中，[3 H]雌二醇或[3 H]孕酮几乎没有代谢，并且在周期的任何阶段，任何一种类固醇的代谢模式都没有改变。在体内的蛋白质合成率在caruncular子宫内膜和峡部输卵管是最高的发情期或后不久（0-2天），然后下降到低水平的第10-14天。这两种组织中的RNA：DNA比值在发情期也最大，随后在第14天降至最小值。结果进行了讨论有关卵巢分泌的雌二醇和孕酮在发情周期的母羊。
: The concentrations of soluble oestradiol and progesterone receptor proteins and several metabolic activities were measured in the genital tracts of ewes killed on Days 0 (oestrus), 2, 5, 10 or 14 of the oestrous cycle. In caruncular endometrium and in whole uterus the concentrations of oestradiol receptor (pmol steroid bound/mg tissue DNA) were highest at oestrus and declined steadily thereafter to minimal values at Day 14. The concentrations of progesterone receptor were highest on Days 0-5, then declined to low levels on Day 10-14. There was little metabolism of either [3H]oestradiol or [3H]progesterone in minces of whole uterus and with either steroid the pattern of metabolism did not change at any stage of the cycle. The in-vivo rates of synthesis of protein in caruncular endometrium and in isthmic oviduct were highest at or shortly after oestrus (Days 0-2), then declined to low levels on Days 10-14. RNA:DNA ratios in these two tissues were also greatest at oestrus and subsequently fell to minimal values by Day 14. The results are discussed in relation to ovarian secretion of oestradiol and progesterone during the oestrous cycle of the ewe.