The complete mitochondrial genome of the scuttle fly, Megaselia scalaris (Diptera: Phoridae)

The complete mitochondrial genome of the scuttle fly, Megaselia scalaris (Diptera: Phoridae)
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DOI:
10.3109/19401736.2013.879651
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发表时间:
2016-01-02
影响因子:
--
通讯作者:
Wen, Jifang
Wen, Jifang
中科院分区:
生物学4区
文献类型:
--
作者:
Zhong, Ming;Wang, Xiang;Wen, Jifang

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蚤蝇属是蚤蝇科中最大的属,全世界有1400多种蚤蝇。首次测定了具有重要医学意义的昆虫--梯阶叶蜂Megaselia scalaris的线粒体全基因组。环状基因组全长15,599 bp,包含典型后生动物基因组中的37个基因:13个蛋白质编码基因,22个转运RNA基因和2个核糖体RNA基因。线粒体基因组还含有一个非编码的A+ T富集区。该基因的排列与其它昆虫的基因排列完全一致。重链上的每种碱基组成如下:A:38.87%,G:13.74%,C:9.46%,T:37.93%和A+T含量76.80%。线粒体基因组的M. scalaris提出的可能是有价值的,以确定系统发育的关系,在双翅目,特别是对家庭Phoridae。这些序列也可作为法医昆虫学物种鉴定的分子标记。
More than 1400 scuttle flies species in worldwide comprise the Megaselia genus, the largest genus in the family Phoridae. The complete mitochondrial genome of Megaselia scalaris, a medically important entomology was sequenced for the first time. The 15,599bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The mitochondrial genome also contains one non-coding A+T-rich region. The arrangement of the genes was identical with other insect. Each of the base composition on heavy strand was as follows A: 38.87%, G: 13.74%, C: 9.46%, T: 37.93% and the A+T content 76.80%. The mitochondrial genome of M. scalaris presented may be valuable for determining phylogenetic relationships within the order Diptera and especially for the family Phoridae. These sequences could also be used to select reliable molecular markers for species identification in forensic entomology.