Rapid isolation of gluten-digesting bacteria from human stool and saliva by using gliadin-containing plates
Rapid isolation of gluten-digesting bacteria from human stool and saliva by using gliadin-containing plates
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DOI：
10.1177/1535370214564748
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发表时间：
2015-07-01
影响因子：
3.2
通讯作者：
Cesario, Thomas
中科院分区：
文献类型：
--
作者：
Berger, Martina;Sarantopoulos, Christos;Cesario, Thomas
在过去的几年里，面筋不耐受患者的数量急剧增加。到目前为止，面筋不耐受的唯一治疗方法是完全避免饮食中的面筋。然而，要维持严格的无麸质饮食是非常具有挑战性的。因此，有必要进行非饮食疗法。任何这样的治疗都必须认识到，面筋的免疫原性部分是醇溶蛋白多肽，它很难被胃肠道的酶降解。益生菌疗法和含有面筋降解菌(GDB)及其醇溶蛋白消化酶的口服酶疗法是治疗面筋不耐受的新方法，但有效地分离GDB用于这些治疗是有问题的。这项研究的目的是开发一种简单的技术来快速有效地分离GDB，希望它可能导致开发出新的方法来开发益生菌或传统药物来治疗面筋不耐受。一些研究人员已经通过使用面筋最少或有限的琼脂平板成功地分离出了GDB。虽然这些平板可以用来分离耐面筋的细菌，但还需要进一步的测试来研究同样的细菌是否也能消化面筋。我们开发的琼脂平板可以检测到既能耐受面筋又能消化面筋的细菌。因此，我们能够将两个步骤结合成一个步骤。利用这些技术，我们能够从唾液和粪便中分离出5株GDB，并鉴定了3株具有面筋降解活性的细菌参比菌株。我们开发的分离具有面筋降解活性的细菌的技术是快速、有效和易于使用的。通过我们的技术分离的GDB可能有潜力作为面筋不耐受患者的益生菌或酶疗法的一部分。
The number of individuals with gluten intolerance has increased dramatically over the last years. To date, the only therapy for gluten intolerance is the complete avoidance of dietary gluten. To sustain a strictly gluten-free diet, however, is very challenging. Therefore, there is need for a non-dietary therapy. Any such treatment must appreciate that the immunogenic part of gluten are gliadin peptides which are poorly degraded by the enzymes of the gastrointestinal tract. Probiotic therapy and oral enzyme therapy containing gluten-degrading bacteria (GDB) and their gliadin-digesting enzymes are possible new approaches for the treatment of gluten intolerance, however effectively isolating GDB for these treatments is problematic. The goal of this study was to develop an easy technique to isolate GDB rapidly and efficiently with the hope it might lead to newer ways of developing either probiotics or traditional medicines to treat gluten intolerance. Several researchers have already isolated successfully GDB by using gluten minimal or limited agar plates. Although these plates can be used to isolate bacteria which can tolerate gluten, further assays are needed to investigate if the same bacteria can also digest gluten. The agar plates we developed can detect bacteria which cannot only tolerate gluten but are able to digest it as well. Therefore, we were able to combine two steps into one step. Using such technologies, we were able to isolate five GDB from saliva and stool, and identified three bacterial reference strains with gluten-degrading activity. The technique we developed to isolate bacteria with gluten-degrading activity is fast, effective, and easy to use. The GDB isolated by our technology could have potential as part of a probiotic or enzymatic therapy for people with gluten intolerance.