Genomic inferences of the cis-regulatory nucleotide polymorphisms underlying gene expression differences between Drosophila melanogaster mating races
Genomic inferences of the cis-regulatory nucleotide polymorphisms underlying gene expression differences between Drosophila melanogaster mating races
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DOI：
10.1093/molbev/msl023
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发表时间：
2006-08-01
影响因子：
10.7
通讯作者：
Wu, Chung-I
中科院分区：
文献类型：
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作者：
Osada, Naoki;Kohn, Michael H.;Wu, Chung-I
影响基因表达的核苷酸序列多态性发生在基因的调控区域（顺式）和基因组的其他地方（反式）。需要进一步的研究来权衡顺式和反式作用突变在介导物种内和物种间基因表达差异方面的相对重要性。在这里，使用微阵列杂交实验分离了 2 个果蝇品系的雌性果蝇头部转录组之间的 363 个基因表达差异。一种菌株（法国）代表世界性的 M 交配种族，另一种菌株（ZS30）代表来自非洲津巴布韦的 Z 交配种族。从这2株菌株改造后的染色体替代菌株中，我们推断M和Z等位基因的表达差异很大程度上归因于差异表达基因所在染色体的基因型，即顺式调控多态性显着影响M和Z之间的基因表达差异。反式调控多态性的影响是明显的，但难以量化。结果对基因调控进化模型以及试图识别果蝇品系之间基因表达差异背后的核苷酸序列多态性的实验研究具有影响。
Nucleotide sequence polymorphisms affecting gene expression occur in the regulatory region of genes (in cis) and elsewhere in the genome (in trans). Further study is required to weigh the relative importance of cis- and trans-acting mutations in mediating gene expression differences within and between species. Here, microarray hybridization experiments were used to isolate 363 gene expression differences between the female fly head transcriptomes of 2 Drosophila melanogaster strains. One strain (French) represented the cosmopolitan M mating race and the other strain (ZS30) represented the Z mating race derived from Zimbabwe, Africa. From chromosomal substitution strains engineered from the 2 strains, we inferred that the expression differences between M and Z alleles largely could be attributed to the genotype of the chromosomes where the differentially expressed genes were located, that is, cis-regulatory polymorphisms prominently influence gene expression differences between M and Z. The effects of trans-regulatory polymorphisms were apparent yet difficult to quantity. Results have implications for models of gene regulatory evolution as well as experimental studies trying to identify the nucleotide sequence polymorphisms underlying gene expression differences between Drosophila strains.