Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non-muscle-invasive bladder cancer (NIMBC).
Copy number aberrations using multicolour fluorescence in situ hybridization (FISH) for prognostication in non-muscle-invasive bladder cancer (NIMBC).
复制标题
使用多色荧光原位杂交 (FISH) 检测拷贝数畸变，用于预测非肌层浸润性膀胱癌 (NIMBC)。
DOI：
10.1111/bju.12232
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Sasaki K.
中科院分区：
文献类型：
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作者：
Matsuyama H;Ikemoto K;Eguchi S;Oga A;Kawauchi S;Yamamoto Y;Kawai Y;Matsumoto H;Hara T;Nagao K;Sakano S;Sasaki K.
目的探讨使用多色荧光原位杂交（FISH）检测染色体3、7、9 p21和17的拷贝数畸变是否可预测非肌层浸润性膀胱癌（NMIBC）患者的预后。（中位年龄50.5岁，男性/女性：91/27，肿瘤1/2/3级：18/52/42，pTis/Ta/T1期：使用UroVysion®试剂盒分析118个样本，以检测染色体3，7，9 p21，当百分比≥ 16%时，变异分数（VF;每条染色体的非模态拷贝数分数之和）定义为异常。9 p21缺失百分比当9 p21位点的拷贝数≥ 12%时，定义为异常，并对我们的队列进行了Maffezzini风险标准分析。（48.3%）患者和12例疾病进展多因素分析显示，9 p21丢失百分比（>12%）是复发的独立预后因素，（P< 0.001，比值比[OR] 3.24，95%可信区间[CI] 1.85-5.62）。单因素分析中，肿瘤分级、尿细胞学阳性、同时发生原位癌和平均VF>16%是影响疾病进展的重要预后因素。在多变量分析中，平均VF>16%是疾病进展的预后因素（P= 0.048，OR 6.07，95%CI 1.02-57.45）。结论使用市售试剂盒进行多色-FISH分析不仅可以成为NMIBC患者诊断和预后的强大工具。
ObjectiveTo investigate if detection of copy number aberrations of chromosomes 3, 7, 9p21, and 17 using multicolour fluorescencein situhybridization (FISH) predicts patient outcome in non‐muscle‐invasive bladder cancer (NMIBC).Patients and MethodsIn all, 118 bladder wash samples were prospectively collected from patients who underwent transurethral resection of bladder tumour (median age 50.5 years, male/female: 91/27, tumour grade 1/2/3: 18/52/42, stage pTis/Ta/T1: 8/62/42) from 2007 to 2010.The 118 samples were analysed using the UroVysion® kit to detect the copy numbers of chromosomes 3, 7, 9p21, and 17.The variant fraction (VF; the sum of the non‐modal copy number fraction of each chromosome) was defined as abnormal when the percentage was ≥16%. The percentage deletion of 9p21 (fraction of null or one copy number of the 9p21 locus) was defined as abnormal when the percentage was ≥12%.Maffezzini risk criteria were also analysed in our cohorts.ResultsThere was recurrence in 57 (48.3%) patients and disease progression in 12 (10.1%), with a median follow‐up of 35.7 months.Multivariate analysis showed that the percentage 9p21 loss (>12%) was an independent prognostic factor for recurrence (P< 0.001, odds ratio [OR] 3.24, 95% confidence interval [CI] 1.85–5.62).For disease progression, tumour grade, positive urine cytology, concurrent carcinomain situ, and a mean VF of >16% were significant prognostic factors in univariate analysis. In multivariate analysis, a mean VF of >16% was a prognostic factor for disease progression (P= 0.048, OR 6.07, 95% CI 1.02–57.45).ConclusionsMulticolour‐FISH analysis using a commercially available kit could be a powerful tool not only for diagnosis, but also for prognostication in patients with NMIBC.