Characterization of two PEBP genes, SrFT and SrMFT, in thermogenic skunk cabbage (Symplocarpus renifolius).
Characterization of two PEBP genes, SrFT and SrMFT, in thermogenic skunk cabbage (Symplocarpus renifolius).
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DOI：
10.1038/srep29440
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发表时间：
2016-07-08
影响因子：
4.6
通讯作者：
Inaba T
中科院分区：
文献类型：
--
作者：
Ito-Inaba Y;Masuko-Suzuki H;Maekawa H;Watanabe M;Inaba T
在许多原始种子植物中都发现了花的产热作用，这些植物的生殖器官在开花期产生热量。因此，表征开花的分子机制，需要充分了解产热的作用，但这方面的产热植物的发展在很大程度上是未知的。本研究通过大量的数据库检索和克隆实验表明，天南星科产热臭鼬甘蓝（Symplocarpus renifolius）具有两个编码磷脂酰乙醇胺结合蛋白（PEBP）的基因，即FLOWERLOCUS T（SrFT）和MOTHER OF FT AND TFL1（SrMFT）。拟南芥SrFT和SrMFT的功能分析表明，SrFT促进开花，而SrMFT不。In S.在肾叶中，SrFT的阶段特异性和组织特异性表达较SrMFT明显。SrFT在花和叶中高表达，主要定位于维管组织。此外，微阵列分析表明，在花组织中，SrFT与细胞呼吸和线粒体功能相关的基因，包括交替氧化酶基因提出发挥主要作用的花产热。综上所述，这些数据表明，在PEBP基因，SrFT发挥作用，在开花和花发育的产热臭鼬菜。
Floral thermogenesis has been found in dozens of primitive seed plants and the reproductive organs in these plants produce heat during anthesis. Thus, characterization of the molecular mechanisms underlying flowering is required to fully understand the role of thermogenesis, but this aspect of thermogenic plant development is largely unknown. In this study, extensive database searches and cloning experiments suggest that thermogenic skunk cabbage (Symplocarpus renifolius), which is a member of the family Araceae, possesses two genes encoding phosphatidyl ethanolamine-binding proteins (PEBP), FLOWERING LOCUS T (SrFT) and MOTHER OF FT AND TFL1 (SrMFT). Functional analyses of SrFT and SrMFT in Arabidopsis indicate that SrFT promotes flowering, whereas SrMFT does not. In S. renifolius, the stage- and tissue-specific expression of SrFT was more evident than that of SrMFT. SrFT was highly expressed in flowers and leaves and was mainly localized in fibrovascular tissues. In addition, microarray analysis revealed that, within floral tissues, SrFT was co-regulated with the genes associated with cellular respiration and mitochondrial function, including ALTERNATIVE OXIDASE gene proposed to play a major role in floral thermogenesis. Taken together, these data suggest that, among the PEBP genes, SrFT plays a role in flowering and floral development in the thermogenic skunk cabbage.