Deterioration of physical performance and cognitive function in rats with short-term high-fat feeding
Deterioration of physical performance and cognitive function in rats with short-term high-fat feeding
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DOI：
10.1096/fj.09-139691
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发表时间：
2009-12-01
期刊：
影响因子：
4.8
通讯作者：
Clarke, Kieran
中科院分区：
文献类型：
--
作者：
Murray, Andrew J.;Knight, Nicholas S.;Clarke, Kieran
效率，定义为消耗给定氧气量时产生的功量，是耐力能力的关键决定因素，并且可以通过代谢底物供应来改变，因为脂肪酸氧化的效率低于葡萄糖氧化。然而，由于所谓的糖原节约特性，目前尚不清楚食用高脂肪饮食是否会对耐力能力有害或有益。此外，几个月的高脂肪饮食会导致认知障碍。在这里，我们测试了这样的假设：与对照食物饮食（7.5% kcal 来自脂肪）相比，短期摄入高脂肪饮食（55% kcal 来自脂肪）会通过线粒体解偶联和能量剥夺损害大鼠的运动能力和认知功能。我们发现，老鼠在跑步机上跑的距离减少了 35%，并且在 9 天高脂肪喂养的迷宫测试中表现出认知障碍，骨骼肌线粒体出现呼吸解偶联，与解偶联蛋白 (UCP3) 水平增加相关。我们的结果表明，高脂肪喂养，即使是在短时间内，也会改变骨骼肌 UCP3 的表达，影响能量产生和身体表现。优化营养以最大限度地提高线粒体 ATP 产生效率可以改善运动员和代谢异常患者的能量。-Murray, A. J.、Knight, N. S.、Cochlin, L. E.、McAleese, S.、Deacon, R. M. J.、Rawlins, J. N. P.、Clarke, K.短期高脂肪喂养大鼠的身体表现和认知功能恶化。 FASEB J. 23, 4353-4360 (2009)。 www.fasebj.org
Efficiency, defined as the amount of work produced for a given amount of oxygen consumed, is a key determinant of endurance capacity, and can be altered by metabolic substrate supply, in that fatty acid oxidation is less efficient than glucose oxidation. It is unclear, however, whether consumption of a high-fat diet would be detrimental or beneficial for endurance capacity, due to purported glycogen-sparing properties. In addition, a high-fat diet over several months leads to cognitive impairment. Here, we tested the hypothesis that short-term ingestion of a high-fat diet (55% kcal from fat) would impair exercise capacity and cognitive function in rats, compared with a control chow diet (7.5% kcal from fat) via mitochondrial uncoupling and energy deprivation. We found that rats ran 35% less far on a treadmill and showed cognitive impairment in a maze test with 9 d of high-fat feeding, with respiratory uncoupling in skeletal muscle mitochondria, associated with increased uncoupling protein (UCP3) levels. Our results suggest that high-fat feeding, even over short periods of time, alters skeletal muscle UCP3 expression, affecting energy production and physical performance. Optimization of nutrition to maximize the efficiency of mitochondrial ATP production could improve energetics in athletes and patients with metabolic abnormalities.-Murray, A. J., Knight, N. S., Cochlin, L. E., McAleese, S., Deacon, R. M. J., Rawlins, J. N. P., Clarke, K. Deterioration of physical performance and cognitive function in rats with short-term high-fat feeding. FASEB J. 23, 4353-4360 (2009). www.fasebj.org