EFFECT OF DIALYSATES AND ULTRAFILTRATES OF PLASMA OF SALINE-LOADED DOGS ON TOAD BLADDER SODIUM TRANSPORT
EFFECT OF DIALYSATES AND ULTRAFILTRATES OF PLASMA OF SALINE-LOADED DOGS ON TOAD BLADDER SODIUM TRANSPORT
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DOI：
10.1172/jci106312
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发表时间：
1970-01-01
影响因子：
15.9
通讯作者：
GREEN, WE
中科院分区：
文献类型：
--
作者：
BUCKALEW, VM;MARTINEZ, FJ;GREEN, WE
为了获得利尿钠激素存在的直接证据，用生理盐水扩张的狗血浆透析液和超滤法检测对蟾蜍膀胱钠转运的影响。通过在临床透析器中体内透析血液和使用微型临床透析器模型体外透析少量血液获得透析液。用选择性分子过滤器制备了超滤剂，透析液和超滤剂分别使蟾蜍膀胱电位差和短路电流分别变化± 1%和± 5%。相比之下，透析液和ultrasound从扩大狗引起的变化，在电位差的-23%和短路电流的-32%，一个非常显着的差异。短路电流的减少发生在3-5分钟内，在10-20分钟内达到最大值。该效果是迅速可逆的，是特定的serenum表面的膀胱，并不能解释的基础上的非特异性改变离子组成或稀释效应。颈静脉血浆超低温处理比股静脉血浆超低温处理对短路电流的降低更明显。数据表明，在盐水负载犬的血浆中存在蟾蜍膀胱钠转运的可透析抑制剂。使用适当选择性的膜进行的超滤研究表明，该因子的分子量小于3000。
In order to obtain direct evidence for the existence of a natriuretic hormone, dialysates and ultrafiltrates of plasma of dogs expanded with saline were tested for effects on sodium transport by the toad urinary bladder. Dialysate was obtained by dialysis of blood in vivo in a clinical dialyzer and by dialysis in vitro of small volumes of blood using a miniature model of the clinical dialyzer. Ultrafiltrates were prepared using selective molecular filters which permit passage of substances on the basis of molecular weight and three dimensional configuration.Dialysates and ultrafiltrates of hydropenic dogs caused a change in toad bladder potential difference of + 1% and in short circuit current of - 5%. In contrast, dialysates and ultrafiltrates from expanded dogs caused a change in potential difference of - 23% and in short circuit current of - 32%, a highly significant difference. Onset of reduction of short circuit current occurred within 3-5 min, reaching a maximum in 10-20 min. The effect was rapidly reversible, was specific for the serosal surface of the bladder, and could not be explained on the basis of nonspecific alterations in ionic composition or by dilutional effects. Ultrafiltrates of jugular vein plasma caused significantly more reduction of short circuit current than ultrafiltrates of femoral vein plasma. The data indicate the presence in plasma of saline-loaded dogs of a dialyzable inhibitor of toad bladder sodium transport. Ultrafiltrate studies using membranes of appropriate selectivity suggest the factor has a molecular weight of less than 3000.