Dietary genistein increased DMBA-induced mammary adenocarcinoma in wild-type, but not ERαKO, mice
Dietary genistein increased DMBA-induced mammary adenocarcinoma in wild-type, but not ERαKO, mice
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DOI：
10.1207/s15327914nc392_11
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发表时间：
2001-01-01
影响因子：
2.9
通讯作者：
MacDonald, RS
中科院分区：
文献类型：
--
作者：
Day, JK;Besch-Williford, C;MacDonald, RS
含有植物源雌激素浓缩物的膳食补充剂越来越多地被消费者用作激素替代疗法、治疗更年期症状和癌症预防的替代品。在野生型（ER α WT）和雌激素受体α敲除（ER α KO）小鼠中研究了膳食染料木黄酮对二甲基苯并[a]蒽（DMBA）诱导的乳腺肿瘤发展的影响。断奶后，ER α WT 和 ER α KO 小鼠饲喂含有 0 或 1 g 金雀异黄素/kg 饮食的基于酪蛋白的饮食。通过口服DMBA和肩胛下植入醋酸甲羟孕酮来诱导肿瘤。在 ER α KO 小鼠中未观察到肿瘤。在 ER α WT 小鼠中，膳食摄入金雀异黄素会影响肿瘤的发展，从而增强乳腺癌的退行性变。食用金雀异黄酮的小鼠表现出恶性乳腺癌，而在喂食对照饮食的小鼠中观察到良性腺瘤。膳食摄入量也受到金雀异黄酮的影响，喂食金雀异黄酮的 ER α WT 和 ER α KO 小鼠消耗的食物较少（p
Dietary supplements containing concentrates of plant-derived estrogens are being increasingly used by consumers as alternatives for hormone replacement therapy, for treatment of menopausal symptoms, and as cancer preventives. The effect of dietary genistein on dimethylbenz[a] anthracene (DMBA)-induced mammary tumor development was investigated in wild-type (ER alpha WT) and estrogen receptor-a knockout (ER alpha KO) mice. ER alpha WT and ER alpha KO mice were fed a casein-based diet containing 0 or 1 g genistein/kg diet from weaning. Tumors were induced by oral administration of DMBA and subscapular implantation of medroxyprogesterone acetate. No tumors were observed in ER alpha KO mice. In ER alpha WT mice, dietary intake of genistein influenced tumor development, enhancing anaplasia of mammary cancer. Mice consuming genistein expressed malignant mammary adenocarcinoma, whereas benign adenomas were observed in mice fed the control diet. Dietary intake was also influenced by genistein, with ER alpha WT and ER alpha KO mice fed genistein consuming less food (p