The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production.
The transmissible nature of the genetic factor in Escherichia coli that controls haemolysin production.
复制标题
大肠杆菌中控制溶血素产生的遗传因子的传播性质。
DOI：
10.1099/00221287-47-1-153
复制
发表时间：
1967
期刊：
影响因子：
--
通讯作者：
S. Halls
中科院分区：
文献类型：
--
作者：
H. Smith;S. Halls
总结：在51株猪源大肠杆菌中，有6株产肠毒素的遗传因子（Ent）可传递给其它大肠杆菌。大肠杆菌、鼠伤寒沙门氏菌和S.在混合培养中通过接合感染猪霍乱弧菌。在这些培养物中，发现受体菌株的高比例的生物体已经接受了Ent，其可能是质粒。一种生物体对Ent的占有是一种稳定的特征;在实验室培养、吖啶黄处理或在猪和小鼠消化道中的居住期间，没有发现生物体失去了Ent。Ent可以独立于F、R、Hly和K88因子进行传播;负责其传播的转移因子与F以及fi + R因子和Hly的转移因子非常相似。Ent+生物体无法从形态学、文化或抗原上与Ent−生物体区分开来。口服含肠毒素的E.大肠杆菌对猪、仔猪和小牛没有明显的不良影响;液体也不影响皮肤渗透性。Ent向S.鼠伤寒沙门氏菌和S.猪霍乱菌对它们的致病性无影响。给予Ent+ E的四只仔猪中的三只。大肠杆菌微生物发生腹泻;同一菌株的三种特定Ent-微生物没有。
SUMMARY: A genetic factor (designated Ent) responsible for enterotoxin production in six of 51 enterotoxigenic strains of Escherichia coli of porcine origin could be transmitted to other strains of E. coli and to Salmonella typhimurium and S. choleraesuis by conjugation in mixed culture. A high proportion of organisms of the recipient strains in these cultures was found to have accepted Ent, which was probably a plasmid. The possession of Ent by an organism was a stable characteristic; no organisms were found to have lost it during either laboratory cultivation, acriflavine treatment, or residence in the alimentary tract of pigs and mice. Ent could be transmitted independently of F, R, Hly and K88 factors; the transfer factor responsible for its transmission closely resembled F and the transfer factors of fi + R factors and Hly. Ent+ organisms could not be differentiated from Ent− ones, morphologically, culturally or antigenically. The oral administration of enterotoxin-containing bacteria-free culture fluid of an Ent+ strain of E. coli had no apparent ill effect on pigs, piglets and calves; neither did the fluid influence skin permeability. The transmission of Ent to S. typhimurium and S. choleraesuis did not affect their pathogenicity. Three of four piglets given Ent+ E. coli organisms developed diarrhoea; three given Ent− organisms of the same strain did not.