Integrative properties of radial oblique Dendrites in hippocampal CA1 pyramidal neurons
Integrative properties of radial oblique Dendrites in hippocampal CA1 pyramidal neurons
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DOI：
10.1016/j.neuron.2006.03.016
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发表时间：
2006-04-20
期刊：
影响因子：
16.2
通讯作者：
Magee, JC
中科院分区：
文献类型：
--
作者：
Losonczy, A;Magee, JC
尽管径向倾斜树突是 CA1 锥体神经元的主要突触输入位点，但对其整合特性知之甚少。我们使用多位点双光子谷氨酸解禁将不同的时空输入模式传递到单个分支，同时记录解禁引起的兴奋性突触后电位和局部 Ca2+ 信号。尽管存在空间聚类，异步输入模式仍呈线性求和，并产生由 NMDA 受体 (NMDAR) 介导的 Ca2+ 信号。适当定时和大小的输入模式（类似于 6 毫秒内的 20 个输入）会由于树突尖峰的启动而产生超线性求和。与同步输入相关的 Ca2+ 信号更大，并通过 NMDAR 和电压门控 Ca2+ 通道 (VGCC) 的流入介导。斜向尖峰是快速 Na+ 尖峰，其持续时间由 NMDAR、VGCC 和瞬态 K+ 电流的同时激活决定。我们的结果表明，各个分支可以充当单个集成隔室。
Although radial oblique dendrites are a major synaptic input site in CA1 pyramidal neurons, little is known about their integrative properties. We have used multisite two-photon glutamate uncaging to deliver different spatiotemporal input patterns to single branches while simultaneously recording the uncaging-evoked excitatory postsynaptic potentials and local Ca2+ signals. Asynchronous input patterns sum linearly in spite of the spatial clustering and produce Ca2+ signals that are mediated by NMDA receptors (NMDARs). Appropriately timed and sized input patterns (similar to 20 inputs within similar to 6 ms) produce a supralinear summation due to the initiation of a dendritic spike. The Ca2+ signals associated with synchronous input were larger and mediated by influx through both NMDARs and voltage-gated Ca2+ channels (VGCCs). The oblique spike is a fast Na+ spike whose duration is shaped by the coincident activation of NMDAR, VGCCs, and transient K+ currents. Our results suggest that individual branches can function as single integrative compartments.