Phenotypic characterization of a photomorphogenic mutant
Phenotypic characterization of a photomorphogenic mutant
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DOI：
10.1111/j.1365-313x.2004.02148.x
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发表时间：
2004-09-01
期刊：
影响因子：
7.2
通讯作者：
Casal, JJ
中科院分区：
文献类型：
--
作者：
Fankhauser, C;Casal, JJ
光可以说是控制植物生长发育的最重要的非生物因子。植物已经进化出复杂的光感应机制来监测光的质量、强度、方向和周期性（日长）的波动。在拟南芥中，通过分子遗传学研究已经确定了三个光感受器家族。UV-A/蓝光受体隐色素和红色/远红色受体光敏色素控制着一系列重叠的反应，包括光周期开花诱导和去黄化。趋光蛋白是一组对UV-A/蓝光的特异性反应的主要光感受器，如趋光性、叶绿体运动和气孔打开。影响光感受器的突变体具有特征性表型。因此，通过进行一套适当的光生物学和遗传学实验，可以确定突变体中特定的发育反应和光感受器途径。在本文中，我们概述了可以进行的主要和最简单的实验，以获得关于给定突变体光生物学缺陷性质的第一个指示。
Light is arguably the most important abiotic factor controlling plant growth and development throughout their life cycle. Plants have evolved sophisticated light-sensing mechanisms to monitor fluctuations in light quality, intensity, direction and periodicity (day length). In Arabidopsis, three families of photoreceptors have been identified by molecular genetic studies. The UV-A/blue light receptors cryptochromes and the red/far-red receptors phytochromes control an overlapping set of responses including photoperiodic flowering induction and de-etiolation. Phototropins are the primary photoreceptors for a set of specific responses to UV-A/blue light such as phototropism, chloroplast movement and stomatal opening. Mutants affecting a photoreceptor have a characteristic phenotype. It is therefore possible to determine the specific developmental responses and the photoreceptor pathway(s) affected in a mutant by performing an appropriate set of photobiological and genetic experiments. In this paper, we outline the principal and easiest experiments that can be performed to obtain a first indication about the nature of the photobiological defect in a given mutant.