DIFFERENTIAL AGE-CHANGE IN THE NUMBERS OF CD4+CD45RA+ AND CD4+CD29+ T-CELL SUBSETS IN HUMAN PERIPHERAL-BLOOD
DIFFERENTIAL AGE-CHANGE IN THE NUMBERS OF CD4+CD45RA+ AND CD4+CD29+ T-CELL SUBSETS IN HUMAN PERIPHERAL-BLOOD
复制标题
DOI：
10.1016/0047-6374(92)90016-7
复制
发表时间：
1992-03-15
影响因子：
5.3
通讯作者：
SATO, K
中科院分区：
文献类型：
--
作者：
UTSUYAMA, M;HIROKAWA, K;SATO, K
从新生儿至102岁年龄范围内的人获得外周血单核细胞，并通过双色流式细胞仪分析T细胞、B细胞和自然杀伤细胞（CD 3、4、5、8、11 B、19、20、21、25、29、45 RA和56）的各种亚群的数量和百分比。与年轻人相比，T细胞（CD 3+或CD 5+细胞）的数量在30岁时显著下降，在30岁至70岁之间保持相对恒定的水平，此后逐渐下降。在T细胞亚群中，CD 4和CD 8阳性细胞均随年龄增长而下降，但后者下降更为明显，显示出与年龄相关的CD 4/CD 8比值增加。最有趣的发现是CD 4 + T细胞的两个亚群中的对比年龄变化;即抑制诱导T细胞（CD 4 + CD 45 RA+幼稚细胞）的亚群随年龄下降，而辅助诱导T细胞（CD 4 + CD 29+记忆细胞）的亚群随年龄增加。CD 20 + B细胞也以与在T细胞中观察到的类似的方式随着年龄的增长而减少。自然杀伤细胞（CD 56）的数量随着年龄的增长而增加。这些变化之间的关系，在外周血白细胞的各种亚群和年龄相关的免疫功能下降进行了讨论。
Peripheral blood mononuclear cells were obtained from people ranging in age from newborn to 102 years old and analyzed by dual color flow cytometer in terms of number and percentage of various subsets of T cells, B cells and natural killer cells (CD3, 4, 5, 8, 11b, 19, 20, 21, 25, 29, 45RA and 56). Numbers of T cells (CD3+ or CD5+ cells) significantly declined at the 3rd decade as compared with those of younger people, stayed at a relatively constant level between the 3rd and the 7th decade and gradually declined thereafter. In T cell subsets, both CD4 and CD8 positive cells decreased with age, but a decrease was more pronounced in the latter, showing an age-related increase of CD4/CD8 ratio. The most interesting finding was a contrasting age-change in two subsets of CD4+ T cells; i.e. a subset of suppressor inducer T cells (CD4+ CD45RA+ naive cells) decreased with age, while a subset of helper inducer T cells (CD4+ CD29+ memory cells) increased with age. CD20+ B cells also decreased with age in a manner similar to that observed in T cells. Natural killer cells (CD56) showed an increase in numbers with age. The relationship between these changes in various subsets of peripheral blood leukocytes and the age-related decline in immune functions has been discussed.