FREQUENCY AMONG ENTEROBACTERIACEAE OF THE DNA-SEQUENCES ENCODING TYPE-1 PILI
FREQUENCY AMONG ENTEROBACTERIACEAE OF THE DNA-SEQUENCES ENCODING TYPE-1 PILI
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DOI：
10.1128/jb.162.2.799-803.1985
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发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
HULL, SI
中科院分区：
文献类型：
--
作者：
BUCHANAN, K;FALKOW, S;HULL, SI
1型菌毛的特征是能凝集禽类或豚鼠红细胞，且这种凝集可被甘露糖抑制，它存在于整个肠杆菌科中。从大肠杆菌菌毛操纵子的一个限制性内切酶消化片段制备了一种放射性标记探针，并用于检测代表肠杆菌科11个属的236种细菌中的同源DNA序列。只有被鉴定为大肠杆菌或志贺氏菌属的菌株表现出同源性。在9个属中观察到甘露糖敏感的血凝现象。探针DNA不与质粒DNA杂交，表明菌毛操纵子位于染色体上。对来自60株大肠杆菌和2株志贺氏菌的限制性核酸酶消化的全细胞DNA的分析表明，大多数菌株内部序列是保守的，但染色体侧翼序列的变化是常见的。
Type 1 pili, characterized by mannose-inhibitable agglutination of fowl or guinea pig erythrocytes, are found throughout the family Enterobacteriaceae. A radiolabeled probe was prepared from a restriction endonuclease-digested fragment of the Escherichia coli pil operon, and was used to detect homologous DNA sequences in 236 bacteria representing 11 genera of Enterobacteriaceae. Only isolates identified as E. coli or Shigella spp. exhibited homology. Mannose-sensitive hemagglutination was observed in 9 genera. Probe DNA did not hybridize to plasmid DNA, indicating a chromosomal location for the pil operon. Analysis of restriction nuclease-digested whole-cell DNA from 60 E. coli and 2 Shigella sp. isolates indicated that internal sequences were conserved in most strains, but that changes in flanking sequences in the chromosome were common.