In vivo assessment of bacteriotherapy on acetaminophen-induced uremic rats.
In vivo assessment of bacteriotherapy on acetaminophen-induced uremic rats.
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DOI：
10.5301/jn.5000129
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发表时间：
2013
影响因子：
3.4
通讯作者：
A. Mandal;Koushika Das;Suchismita Roy;K. C. Mondal;D. Nandi
中科院分区：
文献类型：
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作者：
A. Mandal;Koushika Das;Suchismita Roy;K. C. Mondal;D. Nandi
对乙酰氨基酚是一种常用的解热剂，高剂量时会导致肾小管损伤和尿毒症。细菌疗法提供了一种有前途的方法，以减轻尿毒症中毒的摄入活的微生物能够分解代谢尿毒症溶质在肠道。本研究评估了非致病性土传脲酶阳性细菌巴氏孢子八叠球菌（Sp）作为潜在的尿素靶向成分，这样的“肠透析”配方。方法雄性白化病大鼠24只，随机分为4组：对照组（NC组），腹腔注射蒸馏水，连续7 d;阳性对照组（U组）腹腔注射对乙酰氨基酚500 mg/kg，连续7 d。试验组（UP组）在腹腔内接受500 mg/kg/天的对乙酰氨基酚7天后，以10（9）个细胞/天的剂量给予Sp 5周。载体对照（VC组）仅接受10（9）个细胞/天剂量的Sp，持续5周，而不进行对乙酰氨基酚处理。划痕后采集血液、肾脏、肝脏和粪便样本，进行生化（血液、肾脏和肝脏的尿素、肌酐、丙二醛、超氧化物歧化酶、过氧化氢酶、谷草转氨酶[GOT]和谷丙转氨酶[GPT]）检测。进行了有限的粪便分析。结果U组血尿素氮（尿素、肌酐）及毒性指标（GOT、GPT）升高，抗氧化酶活性降低。与U组相比，UP组血尿素氮及毒性指标明显降低，抗氧化酶明显升高（P<0.05）。SP处理组的SP数量较NC组和U组增加。结论：研究表明，测试的细菌显着降低血液尿素氮水平。
OBJECTIVES Acetaminophen is a commonly used antipyretic agent which, at high doses, causes renal tubular damage and uremia. Bacteriotherapy affords a promising approach to mitigating uremic intoxication by ingestion of live microbes able to catabolize uremic solutes in the gut. The present study evaluates the nonpathogenic soil-borne urease-positive bacterium Sporosarcina pasteurii (Sp) as a potential urea-targeted component for such an "enteric dialysis" formulation. METHODS Twenty-four albino male rats were randomly divided into 4 groups: The control group (group NC) received distilled water intraperitoneally for 7 days. The positive control group (group U) received 500 mg/kg acetaminophen intraperitoneally for 7 days. The tested group (group UP) was administered Sp at a dosage of 10(9) cells/day for 5 weeks, after receiving 500 mg/kg per day of acetaminophen intraperitoneally for 7 days. Vehicle control (group VC) received only Sp at a dosage of 10(9) cells/day for 5 weeks without acetaminophen treatment. Blood, kidney, liver and stool samples were collected after scarification, for biochemical (urea, creatinine, malondialdehyde, superoxide dismutase, catalase, glutamate oxaloacetate transaminase [GOT] and glutamate pyruvate transaminase [GPT] of blood, kidney and liver) tests. Limited fecal analysis was performed. RESULTS Blood urea nitrogen (urea, creatinine) and toxicity indicators (GOT, GPT) were increased, and antioxidant enzymes were decreased in group U. Blood urea nitrogen and toxicity indicators were reduced, and antioxidant enzymes were increased significantly in the group UP (p<0.05) compared with group U. The number of Sp was increased in Sp-treated groups compared with groups NC and U. CONCLUSIONS The study demonstrated that the bacteria tested reduced blood urea nitrogen levels significantly.