Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy
Metabolic-Activity-Based Assessment of Antimicrobial Effects by D2O-Labeled Single-Cell Raman Microspectroscopy
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通过 D2O 标记的单细胞拉曼显微光谱法进行基于代谢活性的抗菌效果评估
DOI：
10.1021/acs.analchem.6b05051
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发表时间：
2017-04-04
影响因子：
7.4
通讯作者：
Xu, Jian
中科院分区：
文献类型：
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作者：
Tao, Yifan;Wang, Yun;Xu, Jian
为了对抗抗生素耐药性的传播，迫切需要以快速和不依赖培养的方式定量评估药物的代谢抑制作用的方法。在这里，使用四种口腔细菌作为模型，我们表明基于重水（D2 O）的单细胞拉曼显微光谱（D2 O-拉曼）可以使用在2040至2300 cm（-1）中的C-D（碳氘振动）带处的拉曼位移探测细菌对不同药物的响应，作为在单细菌细胞分辨率下代谢活性的通用生物标志物。“基于代谢活性的最小抑制浓度”（MIC-MA）定义为药物暴露8小时时中位Δ CD比率低于
To combat the spread of antibiotic resistance, methods that quantitatively assess the metabolism-inhibiting effects of drugs in a rapid and culture-independent manner are urgently needed. Here using four oral bacteria as models, we show that heavy water (D2O)-based single-cell Raman micro- spectroscopy (D2O-Raman) can probe bacterial response to different drugs using the Raman shift at the C-D (carbon deuterium vibration) band in 2040 to 2300 cm(-1) as a universal biomarker for metabolic activity at single-bacterial-cell resolution. The "minimum inhibitory concentration based on metabolic activity" (MIC-MA), defined as the minimal dose under which the median Delta CD-ratio at 8 h of drug exposure is