Molecular characterization and distribution of motilin family receptors in the human gastrointestinal tract
Molecular characterization and distribution of motilin family receptors in the human gastrointestinal tract
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DOI：
10.1007/s00535-005-1739-0
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发表时间：
2006-03
影响因子：
6.3
通讯作者：
E. Takeshita;B. Matsuura;M. Dong;L. Miller;H. Matsui;M. Onji
中科院分区：
文献类型：
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作者：
E. Takeshita;B. Matsuura;M. Dong;L. Miller;H. Matsui;M. Onji
背景胃动素（Motilin）和生长激素释放肽（Ghrelin）是哺乳动物胃肠运动的重要内源性调节因子，分别由胃动素受体（Motilin receptor）和生长激素释放肽受体（Ghrelin receptor）介导。本研究的目的是探讨胃动素和胃饥饿素受体沿着人胃肠道的分布，并建立人胃动素受体的分子性质。MethodsPost尸检和手术的人体组织标本，没有出血，坏死，或肿瘤，从胃肠道的各个部分。我们分析了胃动素和胃饥饿素受体的mRNA表达水平，并检查了它们的分子身份。部分标本也进行了研究，通过免疫组织化学的胃动素和胃饥饿素receptor.ResultsThe长形式的胃动素受体，但不是短的形式，在胃肠道的所有部分，并表示在肌肉中的表达水平高于粘膜。胃动素受体免疫反应存在于肌细胞和肌间神经丛，但不是在粘膜或粘膜下细胞。与此相反，生长激素释放肽受体mRNA的表达相等的胃肠道的所有部分，在粘膜和肌肉layer.ConclusionsBoth胃动素和生长激素释放肽受体表达沿着人的胃肠道，但他们有明显不同的分布方面的水平和层的表达水平。胃动素受体在基因和蛋白质产物水平上沿着整个胃肠道的弥漫性肌肉表达使其成为胃动素药物治疗运动障碍的有用的潜在靶点。
BackgroundMotilin and ghrelin have been recognized as important endogenous regulators of gastrointestinal motor function in mammals, mediated respectively by the motilin receptor and by the closely related ghrelin receptor. The aims of this study were to explore the distribution of motilin and ghrelin receptors along the human gastrointestinal tract and to establish the molecular nature of the human motilin receptor.MethodsPost mortem and surgical human tissue specimens with no hemorrhage, necrosis, or tumor were obtained from various parts of the gastrointestinal tract. We analyzed levels of expression of mRNA for motilin and ghrelin receptors and examined their molecular identities. Portions of some specimens were also studied by immunohistochemistry for expression of the motilin and ghrelin receptor.ResultsThe long form of the motilin receptor, but not the short form, was expressed in all parts of the gastrointestinal tract, and expressed at higher levels in muscle than in mucosa. Motilin receptor immunoreactivity was present in muscle cells and the myenteric plexus, but not in mucosal or submucosal cells. In contrast, ghrelin receptor mRNA was expressed equally in all parts of the gastrointestinal tract, with similar levels of expression in mucosal and muscle layers.ConclusionsBoth the motilin and ghrelin receptors are expressed along the human gastrointestinal tract, but they have clearly distinct distributions in regard to both level and layer. The diffuse muscle expression of the motilin receptor, at both the levels of the gene and the protein product, along the entire gastrointestinal tract makes it a useful potential target for motilide drugs for dysmotility.