Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells
Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells
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DOI：
10.1007/978-1-4939-3724-0_12
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Maitland, Norman J.
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文献类型：
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作者：
Frame, Fiona M.;Pellacani, Davide;Maitland, Norman J.
为了充分探索诸如前列腺癌的复杂实体瘤的生物学，期望与患者组织一起工作。只有通过研究来自组织的细胞，我们才能考虑到患者的变异性和肿瘤的异质性。细胞系长期以来一直被认为是癌症研究的主力，可以认为，当在一组细胞系中考虑时，它们是最有用的，因此考虑到不同细胞系之间的特定突变和表型变化。然而，当将细胞系与从组织培养的原代细胞进行比较时，常常获得非常不同的结果。从患者组织中培养的细胞代表了一种接近患者的模型，应该并且确实产生了临床相关数据。本章旨在说明处理，储存和培养前列腺组织细胞的方法，并描述潜在的用途。
In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.