Comparison of cardiopulmonary response to endogenous nitric oxide inhibition in pigs inhabited at three levels of altitude
Comparison of cardiopulmonary response to endogenous nitric oxide inhibition in pigs inhabited at three levels of altitude
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DOI：
10.1007/s00421-004-1290-3
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发表时间：
2005-01
影响因子：
3
通讯作者：
Z. Ruan;T. Koizumi;A. Sakai;T. Ishizaki;K. Kubo;T. Shibamoto;Zhangang Wang
中科院分区：
文献类型：
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作者：
Z. Ruan;T. Koizumi;A. Sakai;T. Ishizaki;K. Kubo;T. Shibamoto;Zhangang Wang
一氧化氮（NO）在正常和慢性缺氧的肺循环中起着重要的作用。我们研究了内源性一氧化氮合酶（NOS）抑制对生活在三个海拔水平的未麻醉猪肺和全身血管阻力的影响，以评估NO在适应缺氧环境中的作用。三个地区未麻醉的成年雄性猪[日本松本（海拔680 m，n=5）；中国兴（2,300 m,n=5）；和中国马欣（3750 m,n=5）]，用于血管监测。采用非选择性NOS抑制剂nw -硝基精氨酸（NLA, 20 mg/kg）治疗前后，监测肺动脉压（Ppa）和全身动脉压（Psa），测量肺动脉楔压（Pcwp）和心输出量（CO）。肺血管阻力（PVR）和全身血管阻力（SVR）分别为(Ppa−Pcwp)/CO和psa /CO。与海拔基线相关，巴方升高。NLA给药后，所有动物的ppan和pso均升高，CO降低，导致PVR和SVR升高。然而，三组猪的PVR和SVR的增加没有显著差异。因此，内源性NO的产生有助于调节肺血管基础张力，但在成年猪中，低氧性肺动脉高压的发展似乎独立于NO途径。
Nitric oxide (NO) plays an important role for the pulmonary circulation in normal and chronic hypoxia. We examined effects of endogenous nitric oxide synthase (NOS) inhibition on pulmonary and systemic vascular resistance in unanesthetized pigs living at three levels of altitude to evaluate the role of NO in adaptation to a hypoxic environment. Unanesthetized male adult pigs in three areas [Matsumoto, Japan (680 m above sea level,n=5); Xing, China (2,300 m,n=5); and Maxin, China (3,750 m,n=5)] were prepared for vascular monitoring. Pulmonary (Ppa), and systemic artery pressure (Psa) were monitored, and pulmonary artery wedge pressure (Pcwp) and cardiac output (CO) were measured before and after treatment with a non-selective NOS inhibitor, Nw-nitro-l-argine (NLA; 20 mg/kg). Pulmonary vascular resistance (PVR) and systemic vascular resistance (SVR) were (Ppa−Pcwp)/CO andPsa/CO, respectively. Related to altitude baselinePpawas elevated. After NLA administration,PpaandPsaincreased and CO decreased in all animals, resulting in increases in PVR and SVR. However, there were no significant differences in the increase in PVR and SVR in the three groups of pigs. Thus, endogenous NO production contributes to regulate the basal pulmonary vascular tone, but the development of hypoxic pulmonary hypertension appears to be independent of the NO pathway in adult pigs.