Enhanced expression and production of monocyte chemoattractant protein-1 in inflammatory bowel disease mucosa
Enhanced expression and production of monocyte chemoattractant protein-1 in inflammatory bowel disease mucosa
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DOI：
10.1002/jlb.59.6.804
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发表时间：
1996-06-01
影响因子：
5.5
通讯作者：
Doe, WF
中科院分区：
文献类型：
--
作者：
Grimm, MC;Elsbury, SKO;Doe, WF
外周血单核细胞被募集至炎症性肠病的发炎粘膜，但导致其吸引的具体趋化信号尚不清楚。单核细胞趋化蛋白-1 (MCP-1) 是一种具有单核细胞吸引和激活特性的趋化因子，本研究的目的是检查其在炎症性肠病中的表达和产生。原位杂交证明了巨噬细胞中存在 MCP-1 mRNA（其中一些巨噬细胞是最近从循环中招募的，如单核细胞标记 CD14 的共表达所证明），以及发炎粘膜中的平滑肌和内皮细胞中的 mRNA。免疫组织化学研究表明巨噬细胞、平滑肌和内皮细胞产生的 MCP-1 蛋白具有相应的分布。相比之下，在组织学正常的粘膜中发现最低限度的 MCP-1 mRNA 表达和蛋白质。从手术切除的炎症性肠病结肠中分离并通过 Northern 分析进行检查的巨噬细胞表达 MCP-1 mRNA 的频率显着更高（15/24 与 0/19，P
Peripheral blood monocytes are recruited to the inflamed mucosa of inflammatory bowel disease but the specific chemotactic signals responsible for their attraction are not known. Monocyte chemoattractant protein-1 (MCP-1) is a chemokine with patent monocyte attracting and activating properties and the aim of this study was to examine its expression and production in inflammatory bowel disease. In situ hybridization demonstrated mRNA for MCP-1 in macrophages, some of which had been recently recruited from the circulation as demonstrated by their co-expression of the monocyte marker CD14, as well as in smooth muscle and endothelial cells in inflamed mucose, Immunohistochemical studies showed a corresponding distribution of MCP-1 protein production by macrophages, smooth muscle, and endothelial cells. By contrast minimal MCP-1 mRNA expression and protein were found in histologically normal mucosa. Macrophages isolated from surgically resected inflammatory bowel disease colons and examined by Northern analysis expressed MCP-1 mRNA significantly more frequently (15/24 vs. 0/19, P