Metabolites and Changes in Antioxidant Activity of A-Type and B-Type Proanthocyanidin Dimers after Incubation with Rat Intestinal Microbiota
Metabolites and Changes in Antioxidant Activity of A-Type and B-Type Proanthocyanidin Dimers after Incubation with Rat Intestinal Microbiota
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A型和B型原花青素二聚体与大鼠肠道菌群孵育后的代谢和抗氧化活性变化
DOI：
10.1021/acs.jafc.5b03657
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发表时间：
2015-10-21
影响因子：
6.1
通讯作者：
Li, Chun-mei
中科院分区：
文献类型：
--
作者：
Ge, Zhen-zhen;Dong, Xiao-qian;Li, Chun-mei
在体外与大鼠肠道微生物群孵育0-24 h后，比较B型EC二聚体、A型EC二聚体、A型ECG二聚体和A型EGCG二聚体的代谢。在4个原花青素二聚体中检测到一个“二聚体”分解代谢产物（m/z 815.6）。虽然在B型和A型二聚体中均发生C4-C8黄烷间键的早期断裂和C环的还原性断裂，但这两种类型的二聚体的降解途径可能有所不同。在A型EC二聚体的代谢产物中检测到二聚体分解产物Cl和更多的低分子量酚酸，而在B型EC二聚体中未检测到。孵育6 h后，A型EC二聚体的抗氧化能力显著增强，而B型EC二聚体的抗氧化能力下降。结果表明，原花青素二聚体的抗氧化活性的变化后，大鼠肠道菌群的生物转化不仅是结构依赖性，而且还孵育条件依赖性。
Metabolism of B-type EC dimer, A-type EC dimer, A-type ECG dimer, and A-type EGCG dimer was compared in vitro after incubation with rat intestinal microbiota for 0-24 h. A "dimeric" catabolite (m/z 815.6) was detected in four procyanthocyanidin dirners. Although the early cleavage of the C4-C8 interflavan bond and the reductive cleavage of the C-ring occurred in both B-type and A-type dimers, the degradation routes of these two types of dimers might somewhat differ. A dimeric catabolite Cl and more low molecular weight phenolic acids were detected in the metabolites of A-type EC dimer, but not in B-type EC dimer. The antioxidant capabilities of the A-type dimers were enhanced significantly after incubation for 6 h, whereas the antioxidant capacity of B-type EC dimer decreased. The results suggested that changes in antioxidant activity of procyanidin dimers after bioconversion by rat intestinal microbiota were not only structure dependent but also incubation condition dependent.