GROWTH-FACTORS AND CANINE FLEXOR TENDON HEALING - INITIAL STUDIES IN UNINJURED AND REPAIR MODELS
GROWTH-FACTORS AND CANINE FLEXOR TENDON HEALING - INITIAL STUDIES IN UNINJURED AND REPAIR MODELS
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DOI：
10.1016/s0363-5023(05)80284-9
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发表时间：
1995-07-01
影响因子：
1.9
通讯作者：
HERGRUETER, CA
中科院分区：
文献类型：
--
作者：
DUFFY, FJ;SEILER, JG;HERGRUETER, CA
生长因子在各种骨和软组织愈合过程中的作用已经在最近的许多模型中得到了广泛的研究，但对于可能在屈肌腱愈合中发挥作用的特定生长因子却知之甚少。我们使用了一些成熟的蛋白质纯化技术和生物测定方法，从未手术的犬肌腱中分离并部分鉴定了肝素结合生长因子。我们的数据提供了证据表明，碱性成纤维细胞生长因子，一种有效的血管生成生长因子，存在于正常的犬滑膜内屈肌腱中。然后，我们研究了修复的犬屈肌腱，以进一步阐明生长因子在肌腱愈合过程中的作用。绘制了三个修复间隔(3、10和17天)的肝素-琼脂糖洗脱图谱，并与已知的分离生长因子图谱进行了比较。三个修复间隔显示两个洗脱谱峰，与不同量的血小板衍生生长因子和表皮生长因子一致。虽然需要额外的实验来确定各种蛋白质分离，但这些数据提供了令人信服的证据，表明在未损伤和愈合的指屈肌腱中存在各种生长因子。
The role of growth factors in a variety of bone and soft tissue healing processes has been studied extensively in numerous recent models, yet little is known about the specific growth factors that may be playing a role in flexor tendon healing. We used a number of established protein purification techniques and bioassays to isolate and partially characterize a heparin-binding growth factor from unoperated canine tendons. Our data provide evidence that basic fibroblast growth factor, a potent angiogenic growth factor, is present in normal canine intrasynovial flexor tendons. We then studied repaired canine flexor tendons to further elucidate the role of growth factors in the tendon healing process. Heparin-sepharose elution profiles from three repair intervals (3, 10, and 17 days) were graphed and compared to known profiles of isolated growth factors. The three repair intervals demonstrated two elution profile peaks, consistent with varying amounts of platelet-derived growth factor and epidermal growth factor. Although additional experimentation is required to identify definitively the various protein isolates, these data provide compelling evidence that a variety of growth factors are present in uninjured and healing digital flexor tendons.