The expression of soluble guanylate cyclase in the vasculature of rat skeletal muscle
The expression of soluble guanylate cyclase in the vasculature of rat skeletal muscle
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DOI：
10.1679/aohc.72.117
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发表时间：
2009-09-01
影响因子：
--
通讯作者：
Nojyo, Yoshiaki
中科院分区：
文献类型：
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作者：
Fukutani, Tamotsu;Iino, Satoshi;Nojyo, Yoshiaki
在正常和病理条件下，一氧化氮（NO）在骨骼肌组织中具有多种作用。 NO 主要激活可溶性鸟苷酸环化酶 (sGC) 并介导靶细胞中随后的细胞内信号传导。我们试图使用 sGC α 1 和 sGC β 1 抗体的亚型来鉴定大鼠骨骼肌系统中 NO 的靶细胞。免疫组织化学显示，两种抗体将相同的细胞染色为圆形或椭圆形，并具有多个长突起。 sGC 免疫阳性细胞共表达 NG2 硫酸软骨素蛋白聚糖，这是周细胞的标志物。 sGC 免疫阳性细胞与毛细血管相关，并形成具有延长的细胞质突起的细胞网络。抗肌营养不良蛋白染色的肌肉肌膜中未发现 sGC α 1 和 sGC β 1 ，抗突触素检测到的神经肌肉接头也未发现 sGC α 1 和 sGC β 1。基于这些发现，我们得出结论，sGC 免疫反应性特异性分布在毛细血管周细胞中。骨骼肌系统中的周细胞已被证明是 NO 的靶细胞，并参与微血管血流。
Nitric oxide (NO) has various roles in the skeletal musculature in both normal and pathological conditions. NO primarily activates soluble guanylate cyclase (sGC) and mediates subsequent intracellular signaling in target cells. We sought to identify the target cells of NO in the rat skeletal musculature, using subtypes of sGC alpha 1 and sGC beta 1 antibodies. Immunohistochemistry revealed that both antibodies stained the same cells with round or oval shapes, having several long processes. The sGC-immunopositive cells co-expressed NG2 chondroitin sulfate proteoglycan, a marker of pericytes. The sGC-immunopositive cells were associated with capillaries and formed cellular networks with elongated cytoplasmic processes. sGC alpha 1 and sGC beta 1 were not found in muscle sarcolemma that were stained by anti-dystrophin, or neuromuscular junctions, as detected by anti-synaptophysin. Based on these findings, we concluded that sGC immunoreactivity was specifically distributed in capillary pericytes. Pericytes in the skeletal musculature have been shown to be target cells of NO and are involved in the microvascular blood flow.