Pyrethroid metabolism by eleven Helicoverpa armigera P450s from the CYP6B and CYP9A subfamilies
Pyrethroid metabolism by eleven Helicoverpa armigera P450s from the CYP6B and CYP9A subfamilies
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来自 CYP6B 和 CYP9A 亚科的 11 株棉铃虫 P450 的拟除虫菊酯代谢
DOI：
10.1016/j.ibmb.2021.103597
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发表时间：
2021
影响因子：
3.8
通讯作者：
Yidong Wu
中科院分区：
文献类型：
--
作者：
Yu Shi;Qianqian Jiang;Yihua Yang;René Feyereisen;Yidong Wu
CYP6B 和 CYP9A 亚科的鳞翅目 P450 被认为在宿主植物适应和杀虫剂抗性中发挥重要作用。通过挖掘最近的基因组计划，在这些亚家族中发现了越来越多具有高水平序列同一性的旁系同源物和直系同源物。然而，其中大多数的生化功能仍然未知。需要更好地了解 P450 基因的进化及其编码酶的催化能力，以促进宿主植物利用和杀虫剂抗性的研究。在这里，我们重点研究了多面性草食动物棉铃虫中 CYP6B（4 个基因）和 CYP9A（7 个基因）的完整互补。这些P450在Sf9细胞中异源表达并进行功能比较。体外测定表明，除了进化上不同的CYP6B43之外，所有CYP6B和CYP9A P450都可以有效地代谢艾司芬戊酸。鉴定出一种新的 esfvalerate 2'-羟基代谢物，并发现它是 CYP9A12 产生的主要代谢物。所有测试的 P450 仅显示出对艾司氰戊菊酯的低诱导反应。把这些结果来自H.从armigeraP450s的角度来看，来自34个ditrysian物种的158个完整CYP6B和100个完整CYP9A基因是手动策划的。 CYP9A 亚家族比 CYP6B 亚家族分布更广泛，后者表现出巨大的增减，有 10 个物种缺乏 CYP6B 基因。在21号染色体上发现了两个相邻的CYP6B位点，在鳞翅目进化过程中具有不同的命运。 CYP6B 和 CYP9A 基因的多样性和功能冗余对抗性管理和害虫控制策略提出了挑战，因为昆虫可以利用许多 P450 来应对它们遇到的化学胁迫。
Lepidopteran P450s of the CYP6B and CYP9A subfamilies are thought to play important roles in host plant adaptation and insecticide resistance. An increasing number of paralogs and orthologs with high levels of sequence identity have been found in these subfamilies by mining recent genome projects. However, the biochemical function of most of them remains unknown. A better understanding of the evolution of P450 genes and of the catalytic competence of the enzymes they encode is needed to facilitate studies of host plant use and insecticide resistance. Here, we focused on the full complement of CYP6B (4 genes) and CYP9A (7 genes) in the generalist herbivore,Helicoverpa armigera. These P450s were heterologously expressed in Sf9 cells and compared functionally.In vitroassays showed that all CYP6B and CYP9A P450s can metabolize esfenvalerate efficiently, except for the evolutionarily divergent CYP6B43. A new 2′-hydroxy-metabolite of esfenvalerate was identified and found to be the main metabolite produced by CYP9A12. All tested P450s showed only low induction responses to esfenvalerate. To put these results fromH. armigeraP450s in perspective, 158 complete CYP6B and 100 complete CYP9A genes from 34 ditrysian species were manually curated. The CYP9A subfamily was more widespread than the CYP6B subfamily and the latter showed dramatic gains and losses, with ten species lacking CYP6B genes. Two adjacent CYP6B loci were found on chromosome 21, with different fates during the evolution of Lepidoptera. The diversity and functional redundancy of CYP6B and CYP9A genes challenge resistance management and pest control strategies as many P450s are available to insects to cope with chemical stresses they encounter.