A reporter gene system used to study developmental expressionof alternative oxidase and isolate mitochondrial retrograde regulationmutants in Arabidopsis
A reporter gene system used to study developmental expressionof alternative oxidase and isolate mitochondrial retrograde regulationmutants in Arabidopsis
复制标题
用于研究拟南芥替代氧化酶发育表达并分离线粒体逆行调节突变体的报告基因系统
作者：
Jelena Žarković;S. L. Anderson;D. Rhoads
线粒体功能的扰动会导致植物核基因表达的改变。为了研究这种被称为线粒体逆行调控和发育基因表达的反应，建立了含萤火虫荧光素酶基因的转基因拟南芥(COL-0)系，该基因由拟南芥交替氧化酶1a基因的启动子区域(AtAOX1a)控制。转基因和内源基因在幼子叶中的发育诱导水平高于较老的子叶和叶片。对转基因表达的分析表明，这对新生的叶子也是如此。线粒体电子传递链抑制剂抗霉素A(AA)和TCA循环抑制剂单氟乙酸酯(MFA)可同时诱导转基因和内源基因的表达。观察到转基因对抑制剂的比较反应：(A)子叶对AA处理的反应在大小上与叶片的反应不同；(B)MFA处理的子叶的诱导动力学与叶片的诱导动力学有很大的不同；(C)MFA处理的子叶的诱导动力学与AA在叶片和子叶中的诱导动力学都不同。利用该转基因株系进行遗传筛选，筛选出对AA有较大反应的转基因和AtAOX1a诱变突变体。其中一些突变株系的MFA诱变作用显著降低，但有一株突变株系的诱变效果不明显。总之，这些数据提供了遗传证据，表明不同的线粒体扰动诱导AtAOX1a基因是通过不同的、但重叠的信号通路，这些信号通路是组织特有的。
Perturbation of mitochondrial function causes altered nuclear gene expression in plants. To study this response, called mitochondrial retrograde regulation, and developmental gene expression, a transgenic Arabidopsis thaliana (Col-0) line containing a firefly luciferase gene controlled by a promoter region of the Arabidopsis alternative oxidase 1a gene (AtAOX1a) was created. The transgene and the endogenous gene were developmentally induced in young cotyledons to a level higher than in older cotyledons and leaves. Analysis of transgene expression suggests that this is true for emerging leaves as well. Antimycin A (AA), a mitochondrial electron transport chain inhibitor, and monofluroacetate (MFA), a TCA cycle inhibitor, induced expression of the transgene and the endogenous gene in parallel. The following comparative responses of the transgene to inhibitors were observed: (a) the response in cotyledons to AA treatment differed greatly in magnitude from the response in leaves; (b) the induction kinetics in cotyledons following MFA treatment differed greatly from the kinetics in leaves; and (c) the induction kinetics following MFA treatment differed from the kinetics of AA in both leaves and cotyledons. The transgenic line was used in a genetic screen to isolate mutants with greatly decreased transgene and AtAOX1a induction in response to AA. Some of these mutant lines showed greatly decreased induction by MFA, but one did not. Taken altogether, the data provide genetic evidence that suggests that induction of the AtAOX1a gene by distinct mitochondrial perturbations are via distinct, but overlapping signaling pathways that are tissue specific.
氟乙酸盐处理抑制灌注大鼠肝脏中的混合功能氧化。
影响因子：
5.8
作者：
Zhou,JL;Kauffman,FC;Ballow,CH;Thurman,RG
通讯作者：
Thurman,RG
DOI：
10.1105/tpc.6.12.1747
发表时间：
1994-12
期刊：
The Plant cell
影响因子：
--
作者：
C. Subbaiah;Douglas S. Bush;Martin M. Sachs
通讯作者：
C. Subbaiah;Douglas S. Bush;Martin M. Sachs