Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters
Magnetic resonance imaging of umbilical cord stem cells labeled with superparamagnetic iron oxide nanoparticles: effects of labelling and transplantation parameters
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DOI：
10.1038/s41598-020-70291-9
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发表时间：
2020-08-13
影响因子：
4.6
通讯作者：
Fukuchi, Kazuki
中科院分区：
文献类型：
--
作者：
Ohki, Akiko;Saito, Shigeyoshi;Fukuchi, Kazuki
利用磁共振成像技术对移植细胞的生物分布进行评估是非常重要的。脐带间充质干细胞（UC-MSCs）已成为再生医学中一种很有前途的治疗工具。我们研究了超顺磁性氧化铁（SPIO）和超小超顺磁性氧化铁（USPIO）标记的UC-MSCs在体外和体内的细胞功能和成像效率。UC-MSCs与50或100 μ g/mL标记物浓度的SPIO或USPIO共孵育。分别用台盼蓝染色法和MTT法测定细胞活力和增殖能力。诱导分化（软骨形成、成骨和脂肪形成）来检查标记对干细胞的影响。在体外实验中，我们使用7-T MRI来评估含有不同浓度细胞悬液的幻影的T-2值。活体实验将9只新生大鼠分为对照组、SPIO组和USPIO组。UC-MSCs被直接注射到大鼠脑内。分别于注射后第7天和第14天获得MRI图像。孵育24小时后，UC-MSCs成功地用SPIO和USPIO标记。标记未改变细胞活力。然而，用100 μ g/mL USPIO标记可显著降低细胞增殖。100 μ g/mL的SPIO对软骨分化能力有影响。MRI显示，与未标记的对照细胞不同，标记的细胞表现出清晰的低信号。在uspio标记的细胞中，P
Cell tracking with magnetic resonance imaging (MRI) is important for evaluating the biodistribution of transplanted cells. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have emerged as a promising therapeutic tool in regenerative medicine. We examined the UC-MSCs labeled with superparamagnetic (SPIO) and ultrasmall superparamagnetic iron oxide (USPIO) in terms of cell functioning and imaging efficiency in vitro and in vivo. The UC-MSCs were co-incubated with SPIO or USPIO at a concentration of 50 or 100 mu g/mL of label. Viability and proliferation were assessed by Trypan blue dye exclusion and MTT assay, respectively. Differentiation (chondrogenesis, osteogenesis, and adipogenesis) was induced to examine the impact of labelling on stemness. For in vitro experiments, we used 7-T MRI to assess the T-2 values of phantoms containing various concentrations of cell suspensions. For in vivo experiments, nine neonatal rats were divided into the control, SPIO, and USPIO groups. The UC-MSCs were injected directly into the rat brains. MRI images were obtained immediately and at 7 and 14 days post injection. The UC-MSCs were successfully labeled with SPIO and USPIO after 24 h of incubation. Cell viability was not changed by labelling. Nevertheless, labelling with 100 mu g/mL USPIO led to a significant decrease in proliferation. The capacity for differentiation into cartilage was influenced by 100 mu g/mL of SPIO. MRI showed that labeled cells exhibited clear hypointense signals, unlike unlabeled control cells. In the USPIO-labeled cells, a significant (P