Saccharopolyspora antimicrobica sp nov., an actinomycete from soil
Saccharopolyspora antimicrobica sp nov., an actinomycete from soil
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Saccharopolyspora antimicrobica sp nov.，一种来自土壤的放线菌
DOI：
10.1099/ijs.0.65532-0
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Zhang, Yue-Qin
中科院分区：
文献类型：
--
作者：
Yuan, Li-Jie;Zhang, Yu-Qin;Zhang, Yue-Qin
从中国北京和四川的不同土壤样品中分离到3株革兰氏阳性、好氧、不运动、不耐酸、不耐酒精的菌株，命名为I 05 -00051、I 05 -00074（T）和I 03 -00808。16 S rRNA基因序列分析和DNA-DNA杂交结果表明，3个分离株均为同一基因种。这3个菌株与已知的多孢菌属模式菌株的16 S rRNA基因序列相似性均小于97.0%，但毛多孢菌亚种（Hirsuta subsp.）hirsuta DSM 43463（T）（98.1%基因序列相似性）和刺多孢菌DSM 44228（T）（98.0%相似性）。化学分类学数据，包括内消旋二氨基庚二酸作为诊断性二氨基酸，阿拉伯糖和半乳糖作为主要糖，异-C-15：0、异-C-16：0、异-C-17：0和anteiso-C-17：0作为主要脂肪酸，MK-9（H-4）作为主要甲基萘醌，极性脂质主要由二磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油和磷脂酰肌醇组成，支持这三种生物隶属于多孢菌属。基因组DNA G + C含量为68.2- 69.9mol%。通过DNA-DNA杂交实验，证明了这3个菌株与S。多毛亚种hirsuta DSM 43463（T）和S. spinosaDSM 44228（T），结合化学分类学和生理学数据，证明了这三个新的分离物代表了多孢菌属的一个新种，建议将其命名为抗微生物多孢菌新种。模式菌株为105-00074（T）（= CCM 7463（T）= KCTC 19303（T））。
Three Gram-positive, aerobic, non-motile, non-acid-alcohol-fast strains, designated I05-00051, I05-00074(T) and I03-00808, were isolated from different soil samples in Beijing and Sichuan, China. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridization experiments revealed that these three isolates represented the same genospecies. These three strains showed < 97.0% 16S rRNA gene sequence similarity with the type strains of recognized species of the genus Saccharopolyspora, with the exception of Saccharopolyspora hirsuta subsp. hirsuta DSM 43463(T) (98.1% gene sequence similarity) and Saccharopolyspora spinosa DSM 44228(T) (98.0% similarity). Chemotaxonomic data, including meso-diaminopimelic acid as the diagnostic diamino acid, arabinose and galactose as predominant sugars, iso-C-15:0, iso-C-16:0, iso-C-17:0 and anteiso-C-17:0 as major fatty acids, MK-9(H-4) as predominant menaquinone and polar lipids dominated by diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol, supported the affiliation of these three organisms to the genus Saccharopolyspora. The genomic DNA G + C contents of the three isolates were 68.2-69.9 mol%. The results of DNA-DNA hybridization experiments among these three isolates and S. hirsuta subsp. hirsuta DSM 43463(T) and S. spinosa DSM 44228(T), in combination with chemotaxonomic and physiological data, demonstrated that the three new isolates represent a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora antimicrobica sp. nov. is proposed. The type strain is 105-00074(T) (= CCM 7463(T) = KCTC 19303(T)).