Detecting specific infections in children through host responses: a paradigm shift.
Detecting specific infections in children through host responses: a paradigm shift.
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DOI：
10.1097/qco.0000000000000065
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发表时间：
2014-06
影响因子：
3.9
通讯作者：
Ramilo O
中科院分区：
文献类型：
--
作者：
Mejias A;Suarez NM;Ramilo O
There is a need for improved diagnosis and for optimal classification of patients with infectious diseases. An alternative approach to the pathogen-detection strategy is based on a comprehensive analysis of the host response to the infection. This review focuses on the value of transcriptome analyses of blood leukocytes for the diagnosis and management of patients with infectious diseases. Initial studies showed that RNA from blood leukocytes of children with acute viral and bacterial infections carried pathogen-specific transcriptional signatures. Subsequently, transcriptional signatures for several other infections have been described and validated in humans with malaria, dengue, salmonella, meloidosis, RSV, influenza, tuberculosis and HIV. In addition, transcriptome analyses represent an invaluable tool to understand disease pathogenesis, and to objectively classify patients according to clinical severity. Microarray studies have shown to be highly reproducible using different platforms, and in different patient populations, confirming the value of blood transcriptome analyses to study pathogen-specific host immune responses in the clinical setting. Combining the detection of the pathogen with a comprehensive assessment of the host immune response will provide a new understanding of the correlations between specific etiologic agents, the host response and the clinical manifestations of the disease.
