In Vivo Nanotoxicity Testing using the Zebrafish Embryo Assay.
In Vivo Nanotoxicity Testing using the Zebrafish Embryo Assay.
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DOI：
10.1039/c3tb20528b
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发表时间：
2013-06-10
期刊：
影响因子：
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通讯作者：
Lammers T
中科院分区：
文献类型：
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作者：
Rizzo LY;Golombek SK;Mertens ME;Pan Y;Laaf D;Broda J;Jayapaul J;Möckel D;Subr V;Hennink WE;Storm G;Simon U;Jahnen-Dechent W;Kiessling F;Lammers T
纳米颗粒越来越多地用于生物医学目的。纳米粒子有许多不同的诊断和治疗应用，但其安全性也经常受到严重关注。鉴于每天都有许多新的纳米材料被开发出来，而且人们对接触纳米颗粒的长期毒理学影响知之甚少，因此迫切需要建立有效的纳米毒性测试方法。斑马鱼（Danio rerio）胚胎试验最近已成为体内纳米毒性筛选的一种有趣的“中间”方法，使系统中的（半）高通量分析比培养细胞更复杂，但同时也比小鼠或大鼠中的大规模生物相容性研究更少“侵入性”和更便宜。斑马鱼胚胎检测在环境科学中相对成熟，但在纳米医学领域尚未得到广泛关注。使用原型聚合物药物载体，基于金的纳米诊断和纳米治疗，以及基于氧化铁的纳米诊断，我们在这里表明，使用斑马鱼胚胎的毒性测试是简单，有效和信息丰富的，并忠实地反映，但显着扩展，基于细胞的毒性测试。因此，我们预计斑马鱼胚胎试验将成为一个流行的未来工具，在体内纳米毒性筛选。
Nanoparticles are increasingly used for biomedical purposes. Many different diagnostic and therapeutic applications are envisioned for nanoparticles, but there are often also serious concerns regarding their safety. Given the fact that numerous new nanomaterials are being developed every day, and that not much is known about the long-term toxicological impact of exposure to nanoparticles, there is an urgent need to establish efficient methods for nanotoxicity testing. The zebrafish (Danio rerio) embryo assay has recently emerged as an interesting ‘intermediate’ method for in vivo nanotoxicity screening, enabling (semi-) high-throughput analyses in a system significantly more complex than cultured cells, but at the same time also less ‘invasive’ and less expensive than large-scale biocompatibility studies in mice or rats. The zebrafish embryo assay is relatively well-established in the environmental sciences, but it has not yet gained wide notice in the nanomedicine field. Using prototypic polymeric drug carriers, gold-based nanodiagnostics and nanotherapeutics, and iron oxide-based nanodiagnostics, we here show that toxicity testing using zebrafish embryos is easy, efficient and informative, and faithfully reflects, yet significantly extends, cell-based toxicity testing. We therefore expect that the zebrafish embryo assay will become a popular future tool for in vivo nanotoxicity screening.