Mutations in the Rice OsCHR4 Gene, Encoding a CHD3 Family Chromatin Remodeler, Induce Narrow and Rolled Leaves with Increased Cuticular Wax
Mutations in the Rice OsCHR4 Gene, Encoding a CHD3 Family Chromatin Remodeler, Induce Narrow and Rolled Leaves with Increased Cuticular Wax
复制标题
水稻 OsCHR4 基因突变，编码 CHD3 家族染色质重塑因子，导致叶片狭窄和卷曲，角质层蜡质增加
DOI：
10.3390/ijms20102567
复制
发表时间：
2019-05-02
影响因子：
5.6
通讯作者：
Li, Xueyong
中科院分区：
文献类型：
--
作者：
Guo, Tingting;Wang, Daofeng;Li, Xueyong
叶片叶片宽度，曲率和表皮蜡是大米的重要农艺特征。在这里，我们报告了以多效性表型为特征的米OSCHR4-5突变体，包括狭窄和滚动的叶子，表皮蜡沉积增强，植物高度降低和植物数量。叶片宽度的减小是由纵向静脉数量减少和增长含量增加引起的。在OSCHR4-5突变体中，表皮蜡含量明显更高，从而降低了水流失率和增强的干旱耐受性。分子表征表明，单碱基缺失导致oschr4的第二个染色体域（Chd3（Chdopopase dna结合）DNA结合）家族染色质重塑剂的第二个转移突变，在OSCHR4-5突变体中。在OSCHR4-5 Mutant中上调了七个蜡生物合成基因（GL1-4，WSL4，OSCER7，LACS2，LACS2，ROC4和BDG）和四个Auxin Biosynsis Genes基因（YUC2，YUC3，YUC5和YUC6）的表达。染色质免疫沉淀试验表明，转录活性组蛋白修饰H3K4ME3增加，而在OSCHR4-5突变体中上调的基因中，抑制性H3K27me3降低。因此，OSCHR4通过生长素和蜡生物合成基因表达的表观遗传学调节来调节叶片形态发生和表皮蜡的形成。
Leaf blade width, curvature, and cuticular wax are important agronomic traits of rice. Here, we report the rice Oschr4-5 mutant characterized by pleiotropic phenotypes, including narrow and rolled leaves, enhanced cuticular wax deposition and reduced plant height and tiller number. The reduced leaf width is caused by a reduced number of longitudinal veins and increased auxin content. The cuticular wax content was significantly higher in the Oschr4-5 mutant, resulting in reduced water loss rate and enhanced drought tolerance. Molecular characterization reveals that a single-base deletion results in a frame-shift mutation from the second chromodomain of OsCHR4, a CHD3 (chromodomain helicase DNA-binding) family chromatin remodeler, in the Oschr4-5 mutant. Expressions of seven wax biosynthesis genes (GL1-4, WSL4, OsCER7, LACS2, LACS7, ROC4 and BDG) and four auxin biosynthesis genes (YUC2, YUC3, YUC5 and YUC6) was up-regulated in the Oschr4-5 mutant. Chromatin immunoprecipitation assays revealed that the transcriptionally active histone modification H3K4me3 was increased, whereas the repressive H3K27me3 was reduced in the upregulated genes in the Oschr4-5 mutant. Therefore, OsCHR4 regulates leaf morphogenesis and cuticle wax formation by epigenetic modulation of auxin and wax biosynthetic genes expression.